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Advisor(s)
Abstract(s)
Celiac disease is a gluten-induced autoimmune enteropathy characterized by the presence of tissue tranglutaminase
(tTG) autoantibodies. A disposable electrochemical immunosensor (EI) for the detection
of IgA and IgG type anti-tTG autoantibodies in real patient’s samples is presented. Screen-printed carbon
electrodes (SPCE) nanostructurized with carbon nanotubes and gold nanoparticles were used as the
transducer surface. This transducer exhibits the excellent characteristics of carbon–metal nanoparticle
hybrid conjugation and led to the amplification of the immunological interaction. The immunosensing
strategy consisted of the immobilization of tTG on the nanostructured electrode surface followed by the
electrochemical detection of the autoantibodies present in the samples using an alkaline phosphatase
(AP) labelled anti-human IgA or IgG antibody. The analytical signal was based on the anodic redissolution
of enzymatically generated silver by cyclic voltammetry. The results obtained were corroborated with a
commercial ELISA kit indicating that the electrochemical immunosensor is a trustful analytical screening
tool.
Description
Keywords
Transglutaminase Celiac disease Screen-printed electrodes Carbon nanotubes Gold nanoparticles
Citation
Publisher
Elsevier