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Anthelmintic, Antibacterial and Cytotoxicity Activity of Imidazole Alkaloids fromPilocarpus microphyllusLeaves

2017Journal article Open access
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dc.contributor.authorRocha, Jefferson A.
dc.contributor.authorAndrade, Ivanilza M.
dc.contributor.authorVéras, Leiz M.C.
dc.contributor.authorQuelemes, Patrick V.
dc.contributor.authorLima, David F.
dc.contributor.authorSoares, Maria J.S.
dc.contributor.authorPinto, Pedro L.S.
dc.contributor.authorMayo, Simon J.
dc.contributor.authorIvanova, Galya
dc.contributor.authorRangel, Maria
dc.contributor.authorCorreia, Manuela
dc.contributor.authorMafud, Ana Carolina
dc.contributor.authorMascarenhas, Yvonne P.
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorMoraes, Josué de
dc.contributor.authorEaton, Peter
dc.contributor.authorLeite, José R.S.A.
dc.date.accessioned2019-06-06T10:26:51Z
dc.date.available2019-06-06T10:26:51Z
dc.date.issued2017
dc.description.abstractPilocarpus microphyllus Stapf ex Wardlew (Rutaceae), popularly known as jaborandi, is a plant native to the northern and northeastern macroregions of Brazil. Several alkaloids from this species have been isolated. There are few reports of antibacterial and anthelmintic activities for these compounds. In this work, we report the antibacterial and anthelmintic activity of five alkaloids found in P. microphyllus leaves, namely, pilosine, epiisopilosine, isopilosine, epiisopiloturine and macaubine. Of these, only anthelmintic activity of one of the compounds has been previously reported. Nuclear magnetic resonance, HPLC and mass spectrometry were combined and used to identify and confirm the structure of the five compounds. As regards the anthelmintic activity, the alkaloids were studied using in vitro assays to evaluate survival time and damaged teguments for Schistosoma mansoni adult worms. We found epiisopilosine to have anthelmintic activity at very low concentrations (3.125 μg mL-1 ); at this concentration, it prevented mating, oviposition, reducing motor activity and altered the tegument of these worms. In contrast, none of the alkaloids showed antibacterial activity. Additionally, alkaloids displayed no cytotoxic effect on vero cells. The potent anthelmintic activity of epiisopilosine indicates the potential of this natural compound as an antiparasitic agent. Copyright © 2017 John Wiley & Sons, Ltd.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1002/ptr.5771pt_PT
dc.identifier.issn1099-1573
dc.identifier.urihttp://hdl.handle.net/10400.22/13863
dc.language.isoengpt_PT
dc.publisherWileypt_PT
dc.relationNORTE-07-0162-FEDER-00008pt_PT
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/full/10.1002/ptr.5771pt_PT
dc.subjectPilocarpus microphylluspt_PT
dc.subjectImidazole alkaloidspt_PT
dc.subjectEpiisopilosinept_PT
dc.subjectSchistosoma mansonipt_PT
dc.subjectAnthelmintic activitypt_PT
dc.titleAnthelmintic, Antibacterial and Cytotoxicity Activity of Imidazole Alkaloids fromPilocarpus microphyllusLeavespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage630pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage624pt_PT
oaire.citation.titlePhytotherapy Researchpt_PT
oaire.citation.volume31pt_PT
person.familyNameCorreia
person.familyNameDelerue-Matos
person.givenNameManuela
person.givenNameCristina
person.identifier.ciencia-idBC18-CB0A-E027
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0002-3014-5089
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridD-8725-2013
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id7102145897
person.identifier.scopus-author-id6603741848
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication40094cb1-f7d8-4678-b871-64322eb20f86
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscovery09f6a7bd-2f15-42b0-adc5-04bd22210519

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