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Electrochemical magnetoassay coupled to PCR as a quantitative approach to detect the soybean transgenic event GTS40-3-2 in foods

2016Journal article Restricted access
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dc.contributor.authorManzanares-Palenzuela, C. Lorena
dc.contributor.authorMafra, Isabel
dc.contributor.authorCosta, Joana
dc.contributor.authorBarroso, M. Fátima
dc.contributor.authorde-los-Santos-Álvarez, Noemí
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorOliveira, M. Beatriz P. P.
dc.contributor.authorLobo-Castañón, M. Jesus
dc.contributor.authorLópez-Ruiz, Beatriz
dc.date.accessioned2017-01-11T12:21:25Z
dc.date.embargo2115-12
dc.date.issued2016
dc.description.abstractSimple, cost-effective and reliable tools for the quantification of genetically modified organisms (GMO) in food and feed are highly demanded to enforce labelling legislation in the EU. Herein, we report a novel method for quantitative analysis of genetically modified soybean with the event GTS-40-3-2, also known as Roundup Ready (RR) soybean, using magnetoassays with electrochemical detection, coupled to DNA amplification by end-point polymerase chain reaction (PCR). For the proposed work, two DNA sequences were targeted via hybridisation onto magnetic beads, one specific for the transgenic event and the other for the taxon or species-specific lectin gene. Enzymatic labelling was performed to obtain an electrochemically active product measured by chronoamperometry. By optimising the number of PCR cycles, among other parameters, two magnetoassays coupled to PCR were successfully accomplished and linearity was obtained in the ranges of 53–4425 and 1093–88,496 DNA copies for the event-specific and lectin sequences, respectively. The proposed method provides accurate and precise RR soybean quantitative results, being effectively compared to those obtained by real-time PCR, as the reference method. These findings confirm the suitability of the method as an alternative tool for GMO quantification.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.snb.2015.09.013pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/9210
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.ispartofseriesSensors and Actuators B: Chemical;Vol. 222
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0925400515303221pt_PT
dc.subjectGMOpt_PT
dc.subjectRoundup Ready soybeanpt_PT
dc.subjectElectrochemical DNA detectionpt_PT
dc.subjectPCRpt_PT
dc.subjectBiosensorpt_PT
dc.titleElectrochemical magnetoassay coupled to PCR as a quantitative approach to detect the soybean transgenic event GTS40-3-2 in foodspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1057pt_PT
oaire.citation.startPage1050pt_PT
oaire.citation.titleSensors and Actuators B: Chemicalpt_PT
oaire.citation.volume222pt_PT
person.familyNamede Sá Barroso
person.familyNameDelerue-Matos
person.givenNameMaria de Fátima
person.givenNameCristina
person.identifier.ciencia-idAF16-22E5-5EBD
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0001-9011-5992
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id25631648000
person.identifier.scopus-author-id6603741848
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3d701c85-4580-4614-be6d-5a1abc8cf729
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscovery09f6a7bd-2f15-42b0-adc5-04bd22210519

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