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Optical immunosensor panel using quantum dot-antibody conjugates for highly sensitive detection of carbohydrate antigen 19–9 (CA19-9)
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Orientador(es)
Resumo(s)
This study introduces a fluorescent immunosensor colour panel with antibody-conjugated quantum dots for detecting CA19-9 in human serum. The immunosensors presented decreasing fluorescence with increasing CA19-9 concentrations, with a linear detection range from 0.01 to 501.87 U mL−1 and detection limits of 1.66 × 10⁻⁴, 2.71 × 10⁻⁴, and 5.45 × 10⁻⁴ U mL−1 for the green-, orange-, and red-emitting conjugates in human serum. These limits are well below the 37 U mL−1 cutoff for early pancreatic cancer risk. The immunosensor panel is simple, sensitive, and specific, offering a visual colour readout for rapid CA19-9 detection, making it ideal for point-of-care applications. The results highlight the potential of the developed conjugates as a sensitive fluorescence colour panel for detecting CA19-9 across a wide range in human serum, both below and above the pancreatic cancer cutoff. With just 200 μL of sample and a single pre-filtration step, the assay delivers results in under 15 min, offering a cost-effective and efficient platform for point-of-care use.
Descrição
Palavras-chave
Carbohydrate antigen 19–9 (CA19-9) Pancreatic cancer (PC) Quantum dots (QDs) Fluorescent panel immunosensor
Contexto Educativo
Citação
Oliveira, Daniela, Oliveira, Pedro, Xu, Ana, Rodrigues, Eduarda, Guerreiro, Susana G., Castro, Rafael C., Ribeiro, David S.M., Santos, João L.M. & Piloto, Ana Margarida L. (2025). Optical immunosensor panel using quantum dot-antibody conjugates for highly sensitive detection of carbohydrate antigen 19–9 (CA19-9). Analytica Chimica Acta, 1333. https://doi.org/10.1016/j.aca.2024.343399.
Editora
Elsevier