Center for Innovation in Industrial Engineering and Technology

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Publications

Molecularly imprinted polymer for Mucin 4 (MUC4) biomarker

Publication . Piloto, A.M.L.; Cerqueira, N.; Sales, Goreti

(Introduction) Molecularly imprinted polymers (MIPs) were synthesized around quantum dots (QDs) to detect the protein biomarker MUC4, which is associated with pancreatic cancer (Kauretal.,2022; Mehrotraetal.,2024). In this approach, MIPs serve as biorecognition elements and are conjugated with cadmium telluride QDs to form the sensing system. The fluorescence of these imprinted polymers is quenched as MUC4 concentrations increase, demonstrating a linear response within the range of 0.12 ng/mL to 16.9 ng/mL in human serum. This range is below the pancreatic cancer diagnostic cut-off values (1.2 to 1.3 ng/mL). The MIPs exhibit good stability and selectivity for MUC4 compared to non-imprinted controls. Additionally, theoretical studies provide valuable insights for the prediction of the functional structure of MIPs, enhancing its selectivity for the target, while reducing time and costs associated with laborious benchtop assays, which may provide an effective tool for tailoring MIPs to other biomarkers for clinical diagnosis.

2024Conference object

Open access

Plastic antibody for the diagnosis of acute myocardial infarction

Publication . Ricardo, Jacinta; Martins, Gabriela V.; Moreira, Felismina T.C.

(Introduction) Myoglobin (Myo) is the first cardiac biomarker to be released into the bloodstream after the onset of symptoms of Acute Myocardial Infarction (AMI), allowing earlier detection of this disease. Novel application based on Plastic Antibody that responds to a cardiac biomarker, Myo. Imprint stage with electropolymerization of ortho-phenylenediamine (OPD) in the presence of Myo. Template removal from polymeric matrix digested by trypsin. The biomimetic film was fabricated on platinum screen-printed electrodes (Pt-SPE) modified with electroactive Prussian blue nanocubes (PBNCs).

2024Conference object

Open access

Carbon dots de síntese única para deteção de espécies reativas de oxigénio

Publication . Almeida, Lícia; Sousa, Cátia A.; Duarte, Abel J.

(Introdução) As espécies reativas de oxigénio (reactive oxygen species – ROS), são um conjunto de radicais livres que derivam do oxigénio e provêm da cadeia respiratória. São de importância vital nos organismos vivos em quantidade muito reduzidas, funcionando como neurotransmissores ou vasodilatadores, no entanto, quando em grandes quantidades provocam o stresse oxidativo causador de inúmeras patologias. A deteção ROS é de grande importância em diversos processos biológicos, diagnóstico de doenças e triagem de drogas quimioterápicas, para isso é importante construir unidades de carbon dots (Cdots) que sejam sensores químicos de ROS por mecanismo de extinção de fluorescência. Existem algumas estratégias de construção dos CDots, as mais vantajosas são as sínteses únicas em micro-ondas.

2024Conference object

Open access

Detection of CA19-9 using fluorescent polymer imprinted membranes

Publication . Xu, Ana; Piloto, Ana Margarida L.

(Introduction) The Carbohydrate antigen19-9 (CA19-9) is a biomarker widely used for the diagnosis and monitoring of various types of cancer, especially pancreatic cancer (PC). Molecularly Imprinted Polymers (MIPs) are synthetic materials with recognition sites molecularly designed to bind selectively to a target analyte, providing high specificity and sensitivity. In this work, the use of solid supports such as membranes, was used to imprint the MIPs, allowing the creation of robust and easy-to-manipulate biomimetic sensors for the precise and efficient detection of biomarkers, specifically CA19-9.

2024Conference object

Open access

Paper-based approach for cancer biomarker MMP7 detection using quantum dots and molecularly imprinted polymers

Publication . Oliveira, Pedro; Piloto, Ana Margarida L.

(Introduction) Preliminary investigation using lab-on-paper Molecular Imprinted Polymers (MIPs) paired with Quantum Dots (QDs) to detect Matrix Metalloproteinase-7 (MMP7) using image processing to quantify fluorescence and area with a CMOS sensor reflex camera. MMP7 is critical in pancreatic tumor formation, which has the lowest survival rate among malignant malignancies. High levels of the MMP7 in blood have been associated to a poor prognosis and limited survival in patients. CdTe Quantum Dots (CdTe@MPA QDs) are luminescent nanoparticles with narrow emission spectra, enabling for exceptional sensitive detection of biomarkers.Combination of MIPs with CdTe MPA-capped provide selectivitive for biomiarker.

2024Conference object

Open access