Publication
Optical immunosensor panel using quantum dot-antibody conjugates for highly sensitive detection of carbohydrate antigen 19–9 (CA19-9)
| datacite.subject.fos | Engenharia e Tecnologia | |
| datacite.subject.sdg | 09:Indústria, Inovação e Infraestruturas | |
| dc.contributor.author | Oliveira, Daniela | |
| dc.contributor.author | Oliveira, Pedro | |
| dc.contributor.author | Xu, Ana | |
| dc.contributor.author | Rodrigues, Eduarda | |
| dc.contributor.author | Guerreiro, Susana G. | |
| dc.contributor.author | Castro, Rafael C. | |
| dc.contributor.author | Ribeiro, David S. M. | |
| dc.contributor.author | Santos, João L. M. | |
| dc.contributor.author | Piloto, Ana Margarida L. | |
| dc.date.accessioned | 2025-02-11T12:10:00Z | |
| dc.date.available | 2025-02-11T12:10:00Z | |
| dc.date.issued | 2025 | |
| dc.description.abstract | This study introduces a fluorescent immunosensor colour panel with antibody-conjugated quantum dots for detecting CA19-9 in human serum. The immunosensors presented decreasing fluorescence with increasing CA19-9 concentrations, with a linear detection range from 0.01 to 501.87 U mL−1 and detection limits of 1.66 × 10⁻⁴, 2.71 × 10⁻⁴, and 5.45 × 10⁻⁴ U mL−1 for the green-, orange-, and red-emitting conjugates in human serum. These limits are well below the 37 U mL−1 cutoff for early pancreatic cancer risk. The immunosensor panel is simple, sensitive, and specific, offering a visual colour readout for rapid CA19-9 detection, making it ideal for point-of-care applications. The results highlight the potential of the developed conjugates as a sensitive fluorescence colour panel for detecting CA19-9 across a wide range in human serum, both below and above the pancreatic cancer cutoff. With just 200 μL of sample and a single pre-filtration step, the assay delivers results in under 15 min, offering a cost-effective and efficient platform for point-of-care use. | eng |
| dc.description.sponsorship | The authors acknowledge the Portuguese Foundation for Science and Technology (FCT, I.P.) for funding the project “Following up Cancer Biomarkers with New Biomimetic Optical Systems” (https://doi.org/10.54499/2022.07897.PTDC). Ana Margarida L. Piloto also thanks FCT, I.P., for her grant (SFRH/BPD/116067/2016), co-funded by the European Union’s "Programa Operacional Regional Norte 2020," supported by the European Regional Development Fund (ERDF) and the European Social Fund (ESF). David S. M. Ribeiro acknowledges FCT, I.P., for funding through the DL 57/2016 program – Norma Transitória (DL 57/2016/CP1346/CT0033, DOI 10.54499/DL57/2016/CP1346/CT0033). Rafael C. Castro is grateful to FCT/MCTES and the ESF through NORTE 2020 for his PhD grant (ref. 2020.08465.BD, DOI 10.54499/2020.08465.BD). The authors further acknowledge partial support from FCT through grants UIDB/04730/2020 and UIDP/04730/2020. | |
| dc.identifier.citation | Oliveira, Daniela, Oliveira, Pedro, Xu, Ana, Rodrigues, Eduarda, Guerreiro, Susana G., Castro, Rafael C., Ribeiro, David S.M., Santos, João L.M. & Piloto, Ana Margarida L. (2025). Optical immunosensor panel using quantum dot-antibody conjugates for highly sensitive detection of carbohydrate antigen 19–9 (CA19-9). Analytica Chimica Acta, 1333. https://doi.org/10.1016/j.aca.2024.343399. | |
| dc.identifier.doi | 10.1016/j.aca.2024.343399 | |
| dc.identifier.issn | 0003-2670 | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/29454 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Elsevier | |
| dc.relation | Center for Innovation in Industrial Engineering and Technology | |
| dc.relation | UIDP/04730/2020 | |
| dc.relation.hasversion | https://www.sciencedirect.com/science/article/pii/S0003267024012005?via%3Dihub#kwrds0010 | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Carbohydrate antigen 19–9 (CA19-9) | |
| dc.subject | Pancreatic cancer (PC) | |
| dc.subject | Quantum dots (QDs) | |
| dc.subject | Fluorescent panel immunosensor | |
| dc.title | Optical immunosensor panel using quantum dot-antibody conjugates for highly sensitive detection of carbohydrate antigen 19–9 (CA19-9) | eng |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Center for Innovation in Industrial Engineering and Technology | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04730%2F2020/PT | |
| oaire.citation.title | Analytica Chimica Acta | |
| oaire.citation.volume | 1333 | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| relation.isProjectOfPublication | a21a18a5-dd47-49e2-8af6-3e4c8cfaaeea | |
| relation.isProjectOfPublication.latestForDiscovery | a21a18a5-dd47-49e2-8af6-3e4c8cfaaeea |