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Advisor(s)
Abstract(s)
Fossil fuels combustion is one of the major problems of the 21st century. Biodiesel could be one of the responses to this environmental problem. However, the yield and price of production come up as barriers to a global adoption. This study aimed to implement a strategy to improve the performance of biodiesel producing plants, through the application of arbuscular mycorrhizal fungi (AMF). Plants were inoculated with AMF and grown under controlled environmental conditions. Root staining showed the presence of AMF structures in inoculated plants and their absence in non-inoculated plants. No statistically significant seed biomass was observed between inoculated and control plants of Nicotiana tabacum (Tobacco) and Linum usitatissimum (Flaxseed). Chlorophylls assays showed higher chlorophyll content of non-inoculated L. usitatissimum plants in the first assay (before flowering stage) and no statistically significant results in the second assay (after flowering stage). In N. tabacum, no statistically significant results were obtained in both chlorophylls assays. Genetic evaluation in N. tabacum was performed to assess expression of genes involved in triacylglycerol synthesis (DGAT1, DGAT2 and PDGAT) before and during seed development. Results showed that during seed development inoculated plants overexpressed the studied genes compared to control plants. It was hypothesized that a defense mechanism to assure species survival in inoculated plants was strongly induced resulting in production of seeds with higher reserves, namely triacylglycerols.
Description
Keywords
Arbuscular mycorrhizal fungi Biodiesel Genetic expression
Citation
Mendes, J., Baylina, P., Fernandes, R., & Oliveira, R. S. (2022, outubro 21). Effect of arbuscular mycorrhizal fungi application in biodiesel producing plants. Book of Abstracts of 5th Meeting of Medicinal Biotechnology (5MBtM) and 2nd Iberian Congress on Medicinal Biotechnology, 49. https://paginas.ess.ipp.pt/ebtm/2022/5EBTM_BookOfAbstracts.pdf
Publisher
Escola Superior de Saúde P.Porto