Repository logo
 
Publication

Improving the extraction of Ara h 6 (a peanut allergen) from a chocolatebased matrix for immunosensing detection: Influence of time, temperature and additives

2016Journal article Restricted access
Simple item page
dc.contributor.authorAlves, Rita C.
dc.contributor.authorPimentel, Filipa B.
dc.contributor.authorNouws, Henri P.A.
dc.contributor.authorSilva, Túlio H.B.
dc.contributor.authorOliveira, M. Beatriz P.P.
dc.contributor.authorDelerue-Matos, Cristina
dc.date.accessioned2017-01-26T11:51:40Z
dc.date.embargo2117
dc.date.issued2016
dc.description.abstractThe extraction of Ara h 6 (a peanut allergen) from a complex chocolate-based food matrix was optimized by testing different temperatures, extraction times, and the influence of additives (NaCl and skimmed milk powder) in a total of 36 different conditions. Analyses were carried out using an electrochemical immunosensor. Three conditions were selected since they allowed the extraction of the highest levels of Ara h 6. These extractions were performed using 2 g of sample and 20 ml of Tris-HNO3 (pH = 8) containing: a) 0.1 M NaCl and 2 g of skimmed milk powder at 21 C for 60 min; b) 1 M NaCl and 1 g of skimmed milk powder at 21 C for 60 min; and c) 2 g of skimmed milk powder at 60 C for 60 min. Recoveries were similar or higher than 94.7%. This work highlights the importance to adjust extraction procedures regarding the target analyte and food matrix components.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doihttp://dx.doi.org/10.1016/j.foodchem.2016.09.085pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/9431
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.ispartofseriesFood Chemistry;Vol. 218
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0308814616314686pt_PT
dc.subjectPeanutpt_PT
dc.subjectAllergenpt_PT
dc.subjectAra h 6pt_PT
dc.subjectExtractionpt_PT
dc.subjectChocolatept_PT
dc.subjectFoodpt_PT
dc.subjectBiosensorpt_PT
dc.subjectVoltammetricpt_PT
dc.titleImproving the extraction of Ara h 6 (a peanut allergen) from a chocolatebased matrix for immunosensing detection: Influence of time, temperature and additivespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage248pt_PT
oaire.citation.startPage242pt_PT
oaire.citation.titleFood Chemistrypt_PT
oaire.citation.volume218pt_PT
person.familyNameAlves
person.familyNameDelerue-Matos
person.givenNameRita C.
person.givenNameCristina
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0002-5053-513X
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridI-9081-2012
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id7102691286
person.identifier.scopus-author-id6603741848
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication4b5cf5a1-8445-4d6a-ac30-5fbee0326269
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscovery4b5cf5a1-8445-4d6a-ac30-5fbee0326269

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
ART_HenriNouws_GRAQ_2016.pdf
Size:
713.78 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ISEP – GRAQ – Artigos