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Role of oxidative stress-induced systemic and cavernosal molecular alterations in the progression of diabetic erectile dysfunction

2014Journal article Open access
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dc.contributor.authorCastela, Angela
dc.contributor.authorGomes, Pedro
dc.contributor.authorDomingues, Valentina F.
dc.contributor.authorPaíga, Paula
dc.contributor.authorCosta, Raquel
dc.contributor.authorVendeira, Pedro
dc.contributor.authorCosta, Carla
dc.date.accessioned2015-12-23T12:27:14Z
dc.date.available2015-12-23T12:27:14Z
dc.date.issued2014
dc.description.abstractBackground Erectile dysfunction (ED) is a prevalent complication of diabetes, and oxidative stress is an important feature of diabetic ED. Oxidative stress-induced damage plays a pivotal role in the development of tissue alterations. However, the deleterious effects of oxidative stress in the corpus cavernosum with the progression of diabetes remain unclear. The aim of this study was to evaluate systemic and penile oxidative stress status in the early and late stages of diabetes. Methods Male Wistar streptozotocin-diabetic rats (and age-matched controls) were examined 2 (early) and 8 weeks (late) after the induction of diabetes. Systemic oxidative stress was evaluated by urinary H2O2 and the ratio of circulating reduced/oxidized glutathione (GSH/GSSG). Penile oxidative status was assessed by H2O2 production and 3-nitrotyrosine (3-NT) formation. Cavernosal endothelial nitric oxide synthase (eNOS) was analyzed by quantitative immunohistochemistry. Dual immunofluorescence was also performed for 3-NT and α-smooth muscle actin (α-SMA) and eNOS–α-SMA. Results There was a significant increase in urinary H2O2 levels in both diabetic groups. The plasma GSH/GSSG ratio was significantly augmented in late diabetes. In cavernosal tissue, H2O2 production was significantly increased in late diabetes. Reactivity for 3-NT was located predominantly in cavernosal smooth muscle (SM) and was significantly reduced in late diabetes. Quantitative immunohistochemistry revealed a significant decrease in eNOS levels in cavernosal SM and endothelium in late diabetes. Conclusions The findings indicate that the noxious effects of oxidative stress are more prominent in late diabetes. Increased penile protein oxidative modifications and decreased eNOS expression may be responsible for structural and/or functional deregulation, contributing to the progression of diabetes-associated ED.pt_PT
dc.identifier.doi10.1111/1753-0407.12181pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/7239
dc.language.isoengpt_PT
dc.publisherWileypt_PT
dc.relation.ispartofseriesJournal of Diabetes;Vol. 7, Issue 3
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1111/1753-0407.12181/fullpt_PT
dc.subject3-nitrotyrosinept_PT
dc.subjectDiabetespt_PT
dc.subjectEndothelial nitric oxide synthasept_PT
dc.subjectErectile dysfunctionpt_PT
dc.subjectOxidative stresspt_PT
dc.titleRole of oxidative stress-induced systemic and cavernosal molecular alterations in the progression of diabetic erectile dysfunctionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage401pt_PT
oaire.citation.startPage393pt_PT
oaire.citation.titleJournal of Diabetespt_PT
oaire.citation.volume7pt_PT
person.familyNameDomingues
person.familyNamePaíga
person.givenNameValentina Maria Fernandes
person.givenNamePaula
person.identifier1668889
person.identifier.ciencia-id4E16-791D-6664
person.identifier.ciencia-id331A-5D6A-D3C8
person.identifier.orcid0000-0003-3472-849X
person.identifier.orcid0000-0002-9593-1355
person.identifier.ridQ-4930-2018
person.identifier.scopus-author-id6506638953
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationbe653ab6-34ec-4329-972a-eee990a7ec66
relation.isAuthorOfPublication4a0677dc-0b54-4831-aa9a-4a73f0df911d
relation.isAuthorOfPublication.latestForDiscovery4a0677dc-0b54-4831-aa9a-4a73f0df911d

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