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Chromatographic method for the simultaneous quantification of dapsone and clofazimine in nanoformulations

2018Journal article Open access
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dc.contributor.authorMachado, Sandia
dc.contributor.authorFernandes, Sara
dc.contributor.authorChaves, Luise L.
dc.contributor.authorLima, Sofia A. C.
dc.contributor.authorSilva, Eduarda M. P.
dc.contributor.authorBarreiros, Luisa
dc.contributor.authorReis, Salette
dc.contributor.authorSegundo, Marcela A.
dc.date.accessioned2019-06-27T14:02:34Z
dc.date.available2020-05-02T00:30:29Z
dc.date.issued2018
dc.description.abstractThe low bioavailability and nonspecific distribution of dapsone and clofazimine, commonly applied in combination for the treatment of leprosy, can produce toxic effects. Nanotechnological approaches enhance the delivery of these drugs. Therefore, a high-performance liquid chromatography method was developed for the simultaneous determination of dapsone and clofazimine loaded in nanoformulations for quality control purposes. Chromatographic separation was achieved on a reversed-phase Kinetex core-shell C18 column, followed by spectrophotometric detection at 280 nm. Considering the different physicochemical properties of dapsone and clofazimine, elution was performed in gradient mode using an aqueous acetate buffer (50 mmol/L, pH 4.8) and an increasing acetonitrile content from 27 to 63% v/v at a flow rate of 1.0 mL/min with retention times of 6.2 and 14.0 min, respectively. The method was validated according to the European Medicines Agency guideline and it was found to be specific, accurate (99.6-114.0%), and precise for intra- (RSD ≤ 1.8%) and interday assays (RSD ≤ 12.5%). Both drugs showed stability after 24 h at room temperature and over three freeze-thaw cycles with recoveries ≥86.2%. Low temperature (4°C) in the autosampler caused the precipitation of clofazimine and must be avoided. The validated method was successfully applied in the quantification of both drugs in nanoformulations.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1002/jssc.201800427pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/14110
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationPT2020 UID/QUI/50006/2013pt_PT
dc.relationPOCI/01/0145/FEDER/007265pt_PT
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/full/10.1002/jssc.201800427pt_PT
dc.subjectChromatographypt_PT
dc.subjectCore-shell columnspt_PT
dc.subjectLeprosypt_PT
dc.subjectMolecular Structurept_PT
dc.subjectNanostructurespt_PT
dc.subjectNanoparticlespt_PT
dc.subjectBioavailabilitypt_PT
dc.titleChromatographic method for the simultaneous quantification of dapsone and clofazimine in nanoformulationspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage3388pt_PT
oaire.citation.issue17pt_PT
oaire.citation.startPage3382pt_PT
oaire.citation.titleJournal of Separation Sciencept_PT
oaire.citation.volume41pt_PT
person.familyNameFernandes
person.familyNameBarreiros
person.givenNameSara
person.givenNameLuisa
person.identifier.ciencia-id1C12-D800-38A4
person.identifier.ciencia-id611F-E0C5-0230
person.identifier.orcid0000-0001-7042-1941
person.identifier.orcid0000-0003-3481-5809
person.identifier.ridD-7950-2013
person.identifier.scopus-author-id57203278917
person.identifier.scopus-author-id6508205485
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication6c2ed5f0-c3fd-4559-99a3-b8d78d019b47
relation.isAuthorOfPublication1e66bacc-64de-4ecb-96b7-4c0e366cba57
relation.isAuthorOfPublication.latestForDiscovery1e66bacc-64de-4ecb-96b7-4c0e366cba57

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