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Advisor(s)
Abstract(s)
Ovarian cancer is a malignant neoplasm exclusive to females that develops in the ovaries, female sex organs and epithelial ovarian cancer. It has a high mortality rate and is the seventh leading cause of death in women diagnosed with cancer because it develops silently and diagnostic tests have a low predictive value. Thus, developing more accurate diagnostic tests combined with new anti-tumor therapies advance are needy measure to fight this disease. Mesenchymal stem cells (MSC) are multipotent cells, characterized by high proliferation rate and immunomodulatory, secretory, and paracrine properties. Theis cell type can be obtained from several sources in the human body such as marrow bone, adipose tissue and Umbilical cord (UC). UC stands out for being a source that is easily accessible and obtainable, non-invasive, and contains cells with high proliferation and differentiation rates. It has been verified that these cells may be involved in tumor regulatory processes, exerting their effects on metabolic processes such as cell proliferation, survival, angiogenesis, and in tumor-associated inflammatory and immunosuppressive processes [6]. The present work aimed to evaluate how the secretome of human UC-derived MSC (hUC-MSC) influences the tumor capacity of the epithelial ovarian cancer cell line SKOV-3. Conditioned medium (CM) was obtained from hUC-MSC culture and then tumor cells were incubated with different concentrations of conditioned medium, 100%, 50%, and 25%. Parameters such as viability, motility, and cell interactions were evaluated. From the results obtained it was found that CM decreases cell viability, proliferation, and cell-cell interactions, however it stimulated the motility of SKOV-3 cells. In summary, it is possible to conclude that MC from hUC-MSC presented an antitumor effect and can be used as a possible co-adjuvant therapy in the treatment of ovarian cancer.
Description
Keywords
Mesenchymal stem cells Acelluar therapy Conditioned medium Ovarian cancer
Citation
Cristovão, A., Coelho, P., & Gomes, A. (2022). Impact of umbilical cord mesenchymal stem cells secretome in cancer progression. Book of Abstracts of 5th Meeting of Medicinal Biotechnology (5MBtM) and 2nd Iberian Congress on Medicinal Biotechnology, 34. https://paginas.ess.ipp.pt/ebtm/2022/5EBTM_BookOfAbstracts.pdf
Publisher
Escola Superior de Saúde P.Porto