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Avoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell line

2019-04Journal article Open access
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dc.contributor.authorLuís, Carla
dc.contributor.authorCastaño-Guerrero, Yuselis
dc.contributor.authorSoares, Raquel
dc.contributor.authorSales, Goreti
dc.contributor.authorFernandes, Rúben
dc.date.accessioned2021-10-19T13:49:03Z
dc.date.available2021-10-19T13:49:03Z
dc.date.issued2019-04
dc.description.abstractDoxorubicin (DOXO) is an adjuvant chemotherapy agent and is also commonly used in cell biology research. Cytotoxic assays in cell culture are frequently used in order to stablish drug concentrations that are useful for controlling cell proliferation. One common cytotoxic method used is 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT). Our present research aims to support future studies in engaging MTT assay using DOXO that exhibits a strong red coloration and fluorescence, and so it is assumed that DOXO may interfere with commonly used colorimetric assays such as MTT. The interference of DOXO in the MTT determination was evaluated in a Breast Cancer cell line Michigan Cancer Foundation-7 (MCF-7). The interference was evaluated by means of spectroscopic methods in particular spectrophometry and fluorescence spectroscopy of MTT and DOXO. We postulate that the medium and the MTT reagent itself can interfere on the metabolic activity method, so in order to achieve better results, DMEM was replaced by a neutral buffer like Phosphate-buffered saline (PBS). This protocol may be extremely useful in future studies involving DOXO.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLuís, C., Castaño-Guerrero, Y., Soares, R., Sales, G., & Fernandes, R. (2019). Avoiding the Interference of Doxorubicin with MTT Measurements on the MCF-7 Breast Cancer Cell Line. Methods and Protocols, 2(2), 29. https://www.mdpi.com/2409-9279/2/2/29pt_PT
dc.identifier.doi10.3390/mps2020029pt_PT
dc.identifier.issn2409-9279
dc.identifier.urihttp://hdl.handle.net/10400.22/18737
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2409-9279/2/2/29pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMTTpt_PT
dc.subjectDoxorubicinpt_PT
dc.subjectChemotherapypt_PT
dc.subjectBreast cancerpt_PT
dc.subjectMCF-7pt_PT
dc.titleAvoiding the interference of doxorubicin with MTT measurements on the MCF-7 breast cancer cell linept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage5pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleMethods and Protocolspt_PT
oaire.citation.volume2pt_PT
person.familyNameFernandes
person.givenNameRúben
person.identifier635792
person.identifier.ciencia-id0D1F-4090-E82A
person.identifier.orcid0000-0001-8933-3984
person.identifier.scopus-author-id57640135700
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication917f7713-0a35-4ef0-ae56-0784f7fc2187
relation.isAuthorOfPublication.latestForDiscovery917f7713-0a35-4ef0-ae56-0784f7fc2187

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