| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 95.84 KB | Adobe PDF |
Advisor(s)
Abstract(s)
Enrofloxacin (ENR) is an antimicrobial used both in humans and in food producing species.
Its control is required in farmed species and their surroundings in order to reduce the
prevalence of antibiotic resistant bacteria. Thus, a new biomimetic sensor enrofloxacin is
presented. An artificial host was imprinted in specific polymers. These were dispersed in
2-nitrophenyloctyl ether and entrapped in a poly(vinyl chloride) matrix. The potentiometric
sensors exhibited a near-Nernstian response. Slopes expressing mVΔlog([ENR]/M) varied
within 48–63. The detection limits ranged from 0.28 to 1.01 µg mL 1. Sensors were independent
from the pH of test solutions within 4–7. Good selectivity was observed toward
potassium, calcium, barium, magnesium, glycine, ascorbic acid, creatinine, norfloxacin,
ciprofloxacin, and tetracycline. In flowing media, the biomimetic sensors presented good
reproducibility (RSD of ±0.7%), fast response, good sensitivity (47 mV/Dlog([ENR]/
M), wide linear range (1.0×10-5–1.0×10-3 M), low detection limit (0.9 µg mL-1),
and a stable baseline for a 5×10-2 M acetate buffer (pH 4.7) carrier. The sensors were
used to analyze fish samples. The method offered the advantages of simplicity, accuracy,
and automation feasibility. The sensing membrane may contribute to the development of
small devices allowing in vivo measurements of enrofloxacin or parent-drugs.
Description
Keywords
Enrofloxacin Fia Fish Molecularly-imprinted sensors Potentiometry
Citation
Publisher
Taylor & Francis