| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 5.91 MB | Adobe PDF |
Advisor(s)
Abstract(s)
The Arctic Ocean microbiome represents a rich source of metabolic functions that may include
key biogeochemical transformations (e.g. nitrogen cycle) as well as pathways involved in the
production of secondary metabolites associated with coping strategies. There is still an absence
of studies investigating the metabolic and taxonomic link between microorganisms that are
implicated in key ecological functions, such as nitrogen transformations, and microbial
biosynthetic pathways.
This study aims to fill this knowledge gap by studying the distribution of biosynthetic gene
clusters (BGCs) and nitrogen cycle genes in metagenomes collected from the Arctic Ocean.
Through metagenomic analysis, a total of 99 genomes obtained from metagenomes (MAGs)
were recovered and evaluated at the taxonomic and functional level. Several BGCs were
identified, with Terpenes being the most abundant class. The recovered BGCs are distributed by
ten phyla of the Bacteria and Archaea domains, but Proteobacteria, Actinobacteria and
Bacteroidetes stood out as the most prolific, with BGCs associated with the production of new
compounds. Analysing the 7 most complete MAGs, some revealed the presence of genes
associated with nitrogen transformation. These results suggest that microorganisms involved in
the denitrification process also have potential in terms of the production of secondary
metabolites with possible biotechnological applications. Although the possible link between
these two metabolic pathways is still unknown, the microbiological diversity and functional
richness of the Arctic Ocean is emphasized in this project.
Description
Keywords
Artic Ocean Microbiome Metagenomic analysis Secondary metabolites