Publication
The insula, a key brain area for bladder pain control, is modulated by stress
| dc.contributor.author | Summavielle, Teresa | |
| dc.date.accessioned | 2023-03-20T16:45:06Z | |
| dc.date.available | 2023-03-20T16:45:06Z | |
| dc.date.issued | 2023-02 | |
| dc.description.abstract | Stress may cause or aggravate Bladder Pain Syndrome/Interstitial Cystitis (BPS/IC) symptoms. Thus, stress models are being refined to be used as systemic in counterpoint to bladder centric animal models We hypothesised that different stress insults modulate the insula activity diversely. Two models were used in female Wistar rats, Maternal Deprivation Model (MDM, stress applied to new-borns and studies done at 6M) and Water Avoidance Stress test (WAS, stress is applied to 6M rats for 1h every 10 days and studies carried afterwards). Mechanical pain threshold was evaluated in L6/S1 dermatomes which share the spinal cord segments of bladder sensory innervation. Bladder function was evaluated by cystometry. Then Insulas were removed. One was immersed in RNAlater, homogenized, RNA extracted, and expression of different biomarkers assessed by real-time PCR. The other insula was immunoreacted against microglia markers Iba1 and Cd68. The analysis of positive cells used LASAF and ImageJ software. Morphometric analysis and 3D reconstructions used IMARIS software. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Santos-Pereira, M., Silva, S., Santos-Leite, L., Silva, A. I., Terceiro, A. F., Pinto, C., Canedo, T., Monteiro, F., Summavielle, T., Vicent, K., Treede, R., Nagel, J., Merlos, M., Cruz, F., & Charrua, A. (2023). A0764—The insula, a key brain area for bladder pain control, is modulated by stress. Abstracts EAU23 - 38th Annual EAU Congress, 83, S1080. https://doi.org/10.1016/S0302-2838(23)00805-9 | pt_PT |
| dc.identifier.doi | 10.1016/S0302-2838(23)00805-9 | pt_PT |
| dc.identifier.eissn | 1873-7560 | |
| dc.identifier.issn | 0302-2838 | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/22547 | |
| dc.language.iso | eng | pt_PT |
| dc.publisher | Elsevier | pt_PT |
| dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0302283823008059?via%3Dihub | pt_PT |
| dc.title | The insula, a key brain area for bladder pain control, is modulated by stress | pt_PT |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.citation.title | European Urology, Abstracts EAU23 - 38th Annual EAU Congress | pt_PT |
| oaire.citation.volume | 83, Supplement 1 | pt_PT |
| person.familyName | Summavielle | |
| person.givenName | Teresa | |
| person.identifier | 677706 | |
| person.identifier.ciencia-id | C41E-0816-5C85 | |
| person.identifier.orcid | 0000-0003-2548-6281 | |
| person.identifier.rid | C-9776-2012 | |
| person.identifier.scopus-author-id | 6603092949 | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | conferenceObject | pt_PT |
| relation.isAuthorOfPublication | 207ee2de-85a0-4144-9e7e-b376c600e065 | |
| relation.isAuthorOfPublication.latestForDiscovery | 207ee2de-85a0-4144-9e7e-b376c600e065 |