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Synergistic and antibiofilm properties of ocellatin peptides against multidrug-resistant Pseudomonas aeruginosa

2018Journal article Open access
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dc.contributor.authorBessa, Lucinda J
dc.contributor.authorEaton, Peter
dc.contributor.authorDematei, Anderson
dc.contributor.authorPlácido, Alexandra
dc.contributor.authorVale, Nuno
dc.contributor.authorGomes, Paula
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorLeite, José Roberto Sá
dc.contributor.authorGameiro, Paula
dc.date.accessioned2019-10-29T15:30:36Z
dc.date.available2019-10-29T15:30:36Z
dc.date.issued2018
dc.description.abstractAim:To test ocellatin peptides (ocellatins-PT2-PT6) for antibacterial and antibiofilm activities and synergy with antibiotics against Pseudomonas aeruginosa. Materials & methods: Normal- and checkerboard-broth microdilution methods were used. Biofilm studies included microtiter plate-based assays and microscopic analysis by confocal laser scanning microscopy and atomic force microscopy. Results: Ocellatins were more active against multidrug-resistant isolates of P. aeruginosa than against susceptible strains. Ocellatin-PT3 showed synergy with ciprofloxacin and ceftazidime against multidrug-resistant isolates and was capable of preventing the proliferation of 48-h mature biofilms at concentrations ranging from 4 to 8× the MIC. Treated biofilms had low viability and were slightly more disaggregated. Conclusion: Ocellatin-PT3 may be promising as a template for the development of novel antimicrobial peptides against P. aeruginosa.pt_PT
dc.description.sponsorshipThis work received financial support from the European Union (FEDER funds through COMPETE) and National Funds (FCT, Fundação para a Ciência e Tecnologia), under the Partnership Agreement PT2020 through project UID/QUI/50006/2013-POCI/01/0145/FEDER/007265 (LAQV/REQUIMTE) and from Programa Operacional Regional do Norte (ON.2 – O Novo Norte),under the Quadro de Referencia Estrat ˆ egico Nacional (QREN) and funded by Fundo Europeu de Desenvolvimento Regional ´ (Feder) through projects: NORTE-01-0145-FEDER-000024, NORTE-01-0145-FEDER-000011 and NORTE-07-0161-FEDER-000111.A Placido is grateful to FCT for financial support through the grant SFRH ´ /BD/97995/2013, POPH-QREN-Tipologia 4.1-Formação Avançada and Fundo Social Europeu and Ministerio da Ciência, Tecnologia e Ensino Superior. The authors have no other relevant ˆ affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.2217/fmb-2017-0175
dc.identifier.issn1746-0913
dc.identifier.urihttp://hdl.handle.net/10400.22/14762
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFuture Medicinept_PT
dc.relation.publisherversionhttps://www.futuremedicine.com/doi/pdf/10.2217/fmb-2017-0175pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAMPspt_PT
dc.subjectAntibiofilm Activitypt_PT
dc.subjectAntimicrobial peptidespt_PT
dc.subjectMultidrug-resistant Pseudomonas aeruginosapt_PT
dc.subjectOcellatin peptidespt_PT
dc.subjectOcellatin-PT3pt_PT
dc.subjectSynergypt_PT
dc.titleSynergistic and antibiofilm properties of ocellatin peptides against multidrug-resistant Pseudomonas aeruginosapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F97995%2F2013/PT
oaire.citation.endPage163pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage151pt_PT
oaire.citation.titleFuture Microbiologypt_PT
oaire.citation.volume13pt_PT
oaire.fundingStream5876
oaire.fundingStreamSFRH
person.familyNamePlácido
person.familyNameDelerue-Matos
person.givenNameAlexandra
person.givenNameCristina
person.identifier1621053
person.identifier.ciencia-id1B1A-461B-E8DD
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0003-2706-7777
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridL-3085-2014
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id55232677900
person.identifier.scopus-author-id6603741848
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationaad1737a-79f4-4c05-a480-3c0197877757
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscoveryaad1737a-79f4-4c05-a480-3c0197877757
relation.isProjectOfPublication5c06a623-3499-408c-aef9-0a1f68cb1d02
relation.isProjectOfPublicationdf750fb0-a21e-43b2-8a67-f8607fefefcf
relation.isProjectOfPublication.latestForDiscovery5c06a623-3499-408c-aef9-0a1f68cb1d02

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