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Cry1A(b)16 toxin from Bacillus thuringiensis: Theoretical refinement of three-dimensional structure and prediction of peptides as molecular markers for detection of genetically modified organisms

2017Journal article Open access
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dc.contributor.authorPlácido, Alexandra
dc.contributor.authorCoelho, Andreia
dc.contributor.authorAbreu Nascimento, Lucas
dc.contributor.authorGomes Vasconcelos, Andreanne
dc.contributor.authorBarroso, M. Fátima
dc.contributor.authorRamos-Jesus, Joilson
dc.contributor.authorCosta, Vladimir
dc.contributor.authorLima, Francisco das Chagas Alves
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorRamos, Ricardo Martins
dc.contributor.authorMarani, Mariela M.
dc.contributor.authorLeite, José Roberto de Souza de Almeida
dc.date.accessioned2019-06-06T13:40:23Z
dc.date.available2019-06-06T13:40:23Z
dc.date.issued2017
dc.description.abstractTransgenic maize produced by the insertion of the Cry transgene into its genome became the second most cultivated crop worldwide. Cry gene from Bacillus thuringiensis kurstaki expresses protein derivatives of crystalline endotoxins which confer insect resistance onto the maize crop. Mandatory labeling of processed food containing or made by genetically modified organisms is in force in many countries, so, it is very urgent to develop fast and practical methods for GMO identification, for example, biosensors. In the absence of an available empirical structure of Cry1A(b)16 protein, a theoretical model was effectively generated, in this work, by homology modeling and molecular dynamics simulations based on two available homologous protein structures. Molecular dynamics simulations were carried out to refine the selected model, and an analysis of its global structure was performed. The refined models of Cry1A(b)16 showed a standard fold and structural characteristics similar to those seen in Bacillus thuringiensis Cry1A(a) insecticidal toxin and Bacillus thuringiensis serovar kurstaki Cry1A(c) toxin. After in silico analysis of Cry1A(b)16, two immunoreactive candidate peptides were selected and specific polyclonal antibodies were produced resulting in antibody-peptide interaction. Biosensing devices are expected to be developed for detection of the Cry1A(b) protein as a marker of transgenic maize in food. Proteins 2017; 85:1248-1257. © 2017 Wiley Periodicals, Inc.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1002/prot.25285pt_PT
dc.identifier.issn1248-1257
dc.identifier.urihttp://hdl.handle.net/10400.22/13877
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/epdf/10.1002/prot.25285pt_PT
dc.subjectCry1A(b)16pt_PT
dc.subjectBacillus thuringiensispt_PT
dc.subjectHomology modelingpt_PT
dc.subjectMolecular dynamics simulationpt_PT
dc.titleCry1A(b)16 toxin from Bacillus thuringiensis: Theoretical refinement of three-dimensional structure and prediction of peptides as molecular markers for detection of genetically modified organismspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT
oaire.citation.endPage1257pt_PT
oaire.citation.issue7pt_PT
oaire.citation.startPage1248pt_PT
oaire.citation.titleProteinspt_PT
oaire.citation.volume85pt_PT
oaire.fundingStream5876
person.familyNamede Sá Barroso
person.familyNameDelerue-Matos
person.givenNameMaria de Fátima
person.givenNameCristina
person.identifier.ciencia-idAF16-22E5-5EBD
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0001-9011-5992
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id25631648000
person.identifier.scopus-author-id6603741848
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3d701c85-4580-4614-be6d-5a1abc8cf729
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscovery3d701c85-4580-4614-be6d-5a1abc8cf729
relation.isProjectOfPublication5c06a623-3499-408c-aef9-0a1f68cb1d02
relation.isProjectOfPublication.latestForDiscovery5c06a623-3499-408c-aef9-0a1f68cb1d02

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