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Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach

2015-07Journal article Open access
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dc.contributor.authorAlves, Rita C.
dc.contributor.authorPimentel, Filipa B.
dc.contributor.authorNouws, Henri P. A.
dc.contributor.authorCorrer, Wagner
dc.contributor.authorGonzález-García, María Begoña
dc.contributor.authorOliveira, M. Beatriz P. P.
dc.contributor.authorDelerue-Matos, Cristina
dc.date.accessioned2015-12-22T16:09:24Z
dc.date.available2015-12-22T16:09:24Z
dc.date.issued2015-07
dc.description.abstractA voltammetric biosensor for Ara h 6 (a peanut allergen) detection in food samples was developed. Gold nanoparticle-modified screen-printed carbon electrodes were used to develop a sandwich-type immunoassay using two-monoclonal antibodies. The antibody-antigen interaction was detected through the electrochemical detection of enzymatically deposited silver. The immunosensor presented a linear range between 1 and 100 ng/ml, as well as high precision (inter-day RSD ≤9.8 %) and accuracy (recoveries ≥96.7 %). The detection and quantification limits were 0.27 and 0.88 ng/ml, respectively. It was possible to detect small levels of Ara h 6 in complex food matrices.pt_PT
dc.identifier.doi10.1007/s00216-015-8879-8pt_PT
dc.identifier.issn1618-2650
dc.identifier.urihttp://hdl.handle.net/10400.22/7224
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relation.ispartofseriesAnalytical and Bioanalytical Chemistry;Vol. 407, Issue 23
dc.relation.publisherversionhttp://link.springer.com/article/10.1007/s00216-015-8879-8pt_PT
dc.subjectElectrochemical immunosensorpt_PT
dc.subjectAra h 6pt_PT
dc.subjectVoltammetrypt_PT
dc.subjectScreen-printed carbon electrodespt_PT
dc.subjectAllergenpt_PT
dc.subjectPeanutpt_PT
dc.titleDetection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approachpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage7163pt_PT
oaire.citation.startPage7157pt_PT
oaire.citation.titleAnalytical and Bioanalytical Chemistrypt_PT
oaire.citation.volume407pt_PT
person.familyNameAlves
person.familyNameDelerue-Matos
person.givenNameRita C.
person.givenNameCristina
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0002-5053-513X
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridI-9081-2012
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id7102691286
person.identifier.scopus-author-id6603741848
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication4b5cf5a1-8445-4d6a-ac30-5fbee0326269
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscovery09f6a7bd-2f15-42b0-adc5-04bd22210519

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