Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach

Alves, Rita C.; Pimentel, Filipa B.; Nouws, Henri P. A.; Correr, Wagner; González-García, María Begoña; Oliveira, M. Beatriz P. P.; Delerue-Matos, Cristina

http://hdl.handle.net/10400.22/7224

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Authors

González-García, María Begoña

Oliveira, M. Beatriz P. P.

Delerue-Matos, Cristina

Abstract(s)

A voltammetric biosensor for Ara h 6 (a peanut allergen) detection in food samples was developed. Gold nanoparticle-modified screen-printed carbon electrodes were used to develop a sandwich-type immunoassay using two-monoclonal antibodies. The antibody-antigen interaction was detected through the electrochemical detection of enzymatically deposited silver. The immunosensor presented a linear range between 1 and 100 ng/ml, as well as high precision (inter-day RSD ≤9.8 %) and accuracy (recoveries ≥96.7 %). The detection and quantification limits were 0.27 and 0.88 ng/ml, respectively. It was possible to detect small levels of Ara h 6 in complex food matrices.