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Alzheimer’s disease: Development of a sensitive label-freeelectrochemical immunosensor for detection of amyloid beta peptide

2016Journal article Restricted access
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dc.contributor.authorCarneiro, Pedro
dc.contributor.authorLoureiro, Joana
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorMorais, Simone
dc.contributor.authorPereira, Maria do Carmo
dc.date.accessioned2017-02-02T14:34:03Z
dc.date.embargo2117
dc.date.issued2016
dc.description.abstractIn this work, a highly sensitive label-free immunosensor for detection of the main biomarker of Alzheimer’s disease (AD), amyloid beta 1–42 (A (1–42)), is presented. A gold electrode was modified with a mercaptopropionic acid (MPA) self-assembled monolayer, electrodeposited gold nanoparticles (AuNPs) and a monoclonal antibody mAb DE2B4 to recognize A ; all the relevant experimental variables were optimized. Antibodies were functionalized through chemical modification (thiolation) to promote the antibody immobilization on the AuNPs surface with proper orientation which enabled the direct detection of A (1–42). Scanning electron microscopy, square-wave voltammetry and electrochemical impedance spectroscopy were used to characterize the construction of the biosensor. Using the pro-posed immunosensor, A (1–42) was specifically detected within the linear range of 10–1000 pg mL−1 with a 5.2 pg mL−1 and 17.4 pg mL−1 detection and quantification limit, respectively; recovery values for the tested spiking levels ranged from 90.3 to 93.6%. The immunosensor enables rapid, accurate, precise, reproducible and highly sensitive detection (14.6%reduction mL pg−1) of A with low-cost and opens the possibilities for diagnostic ex vivo applications and research-based in vivo studies.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.snb.2016.07.181pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/9492
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.ispartofseriesSensors and Actuators B: Chemical;Volume 239
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0925400516312254pt_PT
dc.subjectAlzheimer’s diseasept_PT
dc.subjectB-Amyloid peptidept_PT
dc.subjectElectrochemical biosensorpt_PT
dc.subjectSelf-assembled monolayerpt_PT
dc.subjectGold nanoparticlespt_PT
dc.subjectMonoclonal antibodypt_PT
dc.titleAlzheimer’s disease: Development of a sensitive label-freeelectrochemical immunosensor for detection of amyloid beta peptidept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage165pt_PT
oaire.citation.startPage157pt_PT
oaire.citation.titleSensors and Actuators B: Chemicalpt_PT
oaire.citation.volume239pt_PT
person.familyNameDelerue-Matos
person.familyNameMorais
person.givenNameCristina
person.givenNameSimone
person.identifier1598822
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.ciencia-idB111-BBFB-F200
person.identifier.orcid0000-0002-3924-776X
person.identifier.orcid0000-0001-6433-5801
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id6603741848
person.identifier.scopus-author-id7007053747
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication7a9c7d63-a6c7-4159-b12d-b049b8c5c8f9
relation.isAuthorOfPublication.latestForDiscovery09f6a7bd-2f15-42b0-adc5-04bd22210519

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