Publication
Evaluation of the anticancer potential of the macrofungus Pisolithus arhizus
| dc.contributor.advisor | Oliveira, Rui S. | |
| dc.contributor.advisor | Martins, Rosário | |
| dc.contributor.advisor | Vieira, Mónica | |
| dc.contributor.author | Costa, Graça Germana Santos da | |
| dc.date.accessioned | 2025-02-18T15:42:15Z | |
| dc.date.available | 2025-02-18T15:42:15Z | |
| dc.date.issued | 2024-09-12 | |
| dc.date.submitted | 2024-09-12 | |
| dc.description.abstract | Microfungi produce bioactive compounds that can greatly enhance health. Pisolithus arhizus is a species of fungus belonging to the phylum Basidiomycota. It is mainly found in association with the roots of some trees, establishing a mutualistic relantionship with them, facilitating the absorption of nutrientes from the soil in Exchange for carbohydrates produced by the host plant. This microfungus has aroused great interest due to its promising therapeutic properties and bioactive effects. This work aimed to evaluate the anticancer potential of a dichloromethane:metanol (2:1) extract. Crude extracts were prepared from the mycelium and culture médium of P. arhizus. The extracts were subsequently separated into 9 fractions using vacum liquid chromatography. The fractions were tested for cytotoxicity against the colon adenocarcinoma cell lines RKO, human neuroblastoma SH-SY5Y and 3T3-L1 fibroblasts. Cell viability was assessed using the 3-(4,5-dimethyl-2thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) reduction assay. The results reaveled cytotoxicity against the RKO cell line in several fractions, with little or no activity in 3T3-L1 cells. The obtained results support previous conclusions that P. arhizus is a promising source of compounds with anticancer activity. However, more in-depth studies are needed to analyze the chemical compounds originating from the various parts of P. arhizus and investigate their biological and toxicological activities. Exploring the bioactive potential of P. arhizus is critical to developing a complete unerstanding of its therapeutic beneficts. | por |
| dc.identifier.tid | 203853091 | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/29578 | |
| dc.language.iso | eng | |
| dc.rights.uri | N/A | |
| dc.subject | Pisolithus arhizus | |
| dc.subject | Macrofungus | |
| dc.subject | Anticancer potential | |
| dc.subject | Natural compounds | |
| dc.title | Evaluation of the anticancer potential of the macrofungus Pisolithus arhizus | por |
| dc.type | master thesis | |
| dspace.entity.type | Publication |