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Antibiotic resistance in wastewater: Occurrence and fate ofEnterobacteriaceaeproducers of Class A and Class C β-lactamases

2014Journal article Open access
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dc.contributor.authorAmador, Paula P.
dc.contributor.authorFernandes, Rúben
dc.contributor.authorPrudêncio, Cristina
dc.contributor.authorBarreto, Mário P.
dc.contributor.authorDuarte, Isabel M.
dc.date.accessioned2019-07-01T13:33:27Z
dc.date.available2019-07-01T13:33:27Z
dc.date.issued2014
dc.description.abstractAntibiotics have been intensively used over the last decades in human and animal therapy and livestock, resulting in serious environmental and public health problems, namely due to the antibiotic residues concentration in wastewaters and to the development of antibiotic-resistant bacteria. This study aimed to access the contribution of some anthropological activities, namely urban household, hospital and a wastewater treatment plant, to the spread of antibiotic resistances in the treated wastewater released into the Mondego River, Coimbra, Portugal. Six sampling sites were selected in the wastewater network and in the river. The ampicillin-resistant Enterobacteriaceae of the water samples were enumerated, isolated and phenotypically characterized in relation to their resistance profile to 13 antibiotics. Some isolates were identified into species level and investigated for the presence of class A and class C -lactamases. Results revealed high frequency of resistance to the -lactam group, cefoxitin (53.5%), amoxicillin/clavulanic acid combination (43.5%), cefotaxime (22.7%), aztreonam (21.3) cefpirome (19.2%), ceftazidime (16.2%) and to the non--lactam group, trimethoprim/sulfamethoxazol (21.1%), tetracycline (18.2%), followed by ciprofloxacin (14.1%). The hospital effluent showed the higher rates of resistance to all antibiotic, except two (chloramphenicol and gentamicin). Similarly, higher resistance rates were detected in the wastewater treatment plant (WWTP) effluent compared with the untreated affluent. Regarding the multidrug resistance, the highest incidence was recorded in the hospital sewage and the lowest in the urban waste. The majority of the isolates altogether are potentially extended-spectrum -lactamases positive (ESBL(+)) (51.9%), followed by AmpC(+) (44.4%) and ESBL(+)/AmpC(+) (35.2%). The most prevalent genes among the potential ESBL producers were blaOXA (33.3%), blaTEM (24.1%) and blaCTX-M (5.6%) and among the AmpC producers were blaEBC (38.9%), blaFOX (1.9%) and blaCIT (1.9%). In conclusion, the hospital and the WWTP activities revealed to have the highest contribution to the spread of multidrug resistant bacteria in the study area. Such data is important for future management of the environmental and public health risk of these contaminants. This is the first embracing study in the water network of Coimbra region on the dissemination of antibiotic resistance determinants. Moreover, it is also the first report with the simultaneous detection of multiresistant bacteria producers of AmpC and ESBLs -lactamases in aquatic systems in Portugal.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAmador, P. P., Fernandes, R., Prudêncio, C., Barreto, M. P., & Duarte, I. M. (2014). Antibiotic resistance in wastewater: Occurrence and fate ofEnterobacteriaceaeproducers of Class A and Class C β-lactamases. Journal of Environmental Science and Health, Part A, 50(1), 26–39. https://doi.org/10.1080/10934529.2015.964602
dc.identifier.doi10.1080/10934529.2015.964602pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/14185
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherTaylor & Francispt_PT
dc.relationStrategic Project - UI 681 - 2011-2012
dc.relation.publisherversionhttps://www.tandfonline.com/doi/full/10.1080/10934529.2015.964602pt_PT
dc.subjectAnimalspt_PT
dc.subjectAnti-Bacterial Agentspt_PT
dc.subjectEnterobacteriaceaept_PT
dc.subjectHumanspt_PT
dc.subjectPortugalpt_PT
dc.subjectRiverspt_PT
dc.subjectSewagept_PT
dc.subjectWaste Waterpt_PT
dc.subjectbeta-Lactamasespt_PT
dc.subjectbeta-Lactamspt_PT
dc.subjectbeta-Lactam Resistancept_PT
dc.titleAntibiotic resistance in wastewater: Occurrence and fate ofEnterobacteriaceaeproducers of Class A and Class C β-lactamasespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 681 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FAGR%2FUI0681%2F2011/PT
oaire.citation.endPage39pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage26pt_PT
oaire.citation.titleJournal of Environmental Science and Health, Part Apt_PT
oaire.citation.volume50pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameFernandes
person.familyNamePrudêncio
person.givenNameRúben
person.givenNameCristina
person.identifier635792
person.identifier1200571
person.identifier.ciencia-id0D1F-4090-E82A
person.identifier.ciencia-idC81E-F4EE-FADE
person.identifier.orcid0000-0001-8933-3984
person.identifier.orcid0000-0002-9920-936X
person.identifier.scopus-author-id57640135700
person.identifier.scopus-author-id6508057930
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication9e6c397c-62d8-4a40-8ec7-ad05ae0ebcc4
relation.isAuthorOfPublication881a8ad5-ab13-4e49-89f4-08ca61cc81e3
relation.isAuthorOfPublication.latestForDiscovery881a8ad5-ab13-4e49-89f4-08ca61cc81e3
relation.isProjectOfPublication7a88e37c-9d46-441f-a16b-251252b516cd
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