Publication
Tracking a Major Egg Allergen to Assess Commercial Food Label Compliance: Towards a Simple and Fast Immunosensing Device
| dc.contributor.author | Freitas, Maria | |
| dc.contributor.author | del Rio, Mariana | |
| dc.contributor.author | Nouws, Henri | |
| dc.contributor.author | Delerue-Matos, Cristina | |
| dc.date.accessioned | 2023-01-24T15:21:40Z | |
| dc.date.available | 2023-01-24T15:21:40Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | An amperometric immunosensor was developed for the analysis of the major egg-white allergen ovotransferrin (Gal d 3) in commercial food products because the (accidental) intake, skin contact with, and/or inhalation of eggs can lead to severe disorders in allergic individuals. Employing a sandwich-type immunosensing strategy, screen-printed carbon electrodes (SPCE) were biomodified with anti-Gal d 3 (capture) antibodies, and the allergen’s detection was achieved with anti-Gal d 3 antibodies labelled with horseradish peroxidase (HRP). The 3,3′,5,5′-tetramethylbenzidine (TMB)/H2O2 reaction with HRP was used to obtain the electrochemical (amperometric) signal. An attractive assay time of 30 min and a remarkable analytical performance was achieved. The quantification range was established between 55 and 1000 ng·mL−1, with a limit of detection of 16 ng·mL−1. The developed method demonstrated good precision (Vx0 = 5.5%) and provided precise results (CV < 6%). The sensor also detected extremely low amounts (down to 0.010%) of egg. The analysis of seven raw and/or cooked egg and egg-white samples indicated that food processing influences the amount of allergen. Furthermore, to assure the compliance of product labelling with EU legislation, 25 commercial food ingredients/products were analysed. The accuracy of the results was confirmed through an ELISA assay. The stability of the ready-to-use sensing surface for 20 days allows a reduction of the reagents’ volumes and cost. | pt_PT |
| dc.description.sponsorship | This research received financial support from the European Union (FEDER funds through COMPETE POCI-01-0145-FEDER-030735) and National Funds (Fundação para a Ciência e a Tecnologia (FCT)) through the project PTDC/QUI-QAN/30735/2017—TracAllerSens—Electrochemical sensors for the detection and quantification of trace amounts of allergens in food products. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.doi | 10.3390/bios12121109 | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.22/21827 | |
| dc.language.iso | eng | pt_PT |
| dc.publisher | MDPI | pt_PT |
| dc.relation | COMPETE POCI-01-0145-FEDER-030735 | pt_PT |
| dc.relation.publisherversion | https://www.mdpi.com/2079-6374/12/12/1109 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
| dc.subject | Egg white | pt_PT |
| dc.subject | Gal d 3 | pt_PT |
| dc.subject | European legislation | pt_PT |
| dc.subject | Electrochemical biosensor | pt_PT |
| dc.subject | Immunosensor; amperometry | pt_PT |
| dc.subject | Food allergy | pt_PT |
| dc.subject | Commercial food | pt_PT |
| dc.subject | Product label | pt_PT |
| dc.title | Tracking a Major Egg Allergen to Assess Commercial Food Label Compliance: Towards a Simple and Fast Immunosensing Device | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FQUI-QAN%2F30735%2F2017/PT | |
| oaire.citation.issue | 12 | pt_PT |
| oaire.citation.startPage | 1109 | pt_PT |
| oaire.citation.title | Biosensors | pt_PT |
| oaire.citation.volume | 12 | pt_PT |
| oaire.fundingStream | 9471 - RIDTI | |
| person.familyName | Nouws | |
| person.familyName | Delerue-Matos | |
| person.givenName | Hendrikus | |
| person.givenName | Cristina | |
| person.identifier | 1046746 | |
| person.identifier.ciencia-id | BC12-319B-037F | |
| person.identifier.ciencia-id | 9B1A-9CED-6EE3 | |
| person.identifier.ciencia-id | 9A1A-43FB-5C27 | |
| person.identifier.orcid | 0000-0002-0628-2753 | |
| person.identifier.orcid | 0000-0002-1472-8866 | |
| person.identifier.orcid | 0000-0002-3924-776X | |
| person.identifier.rid | D-5249-2013 | |
| person.identifier.rid | D-4990-2013 | |
| person.identifier.scopus-author-id | 7801450247 | |
| person.identifier.scopus-author-id | 6603741848 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
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