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Morphological, toxicological and molecular characterization of a benthic Nodularia isolated from Atlantic estuarine environments

2010Journal article Open access
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dc.contributor.authorLopes, Viviana R.
dc.contributor.authorAntunes, Agostinho
dc.contributor.authorWelker, Martin
dc.contributor.authorMartins, Rosário
dc.contributor.authorVasconcelos, Vítor M.
dc.date.accessioned2021-09-28T08:31:42Z
dc.date.available2021-09-28T08:31:42Z
dc.date.issued2010
dc.description.abstractA polyphasic study of a benthic Nodularia isolate (LEGE06071) from an Atlantic environment, specifically salt pans, was performed. LEGE06071 resembled both type strains of Nodularia sphaerocarpa and Nodularia harveyana, while ACOI00729 (purchased isolate) was identified as N. sphaerocarpa. The length and width of vegetative cells varied from 3.10 to 3.15 mu m and from 3.71 to 4.25 mu m, respectively, while heterocyts were 3.91-4.89 mu m long and 4.20-4.74 mu m, wide. None of the isolates had aerotopes. The sequencing of the 16S rRNA gene from the two Nodularia isolates indicated that they belonged neither to Nodularia spumigena nor N. harveyana. Nodularin and other cyanotoxin synthesis-associated genes could not be detected, nor could nodularin production be detected by ELISA. However, MALDI-TOF analysis of LEGE06071 revealed the presence of other compounds, namely, glycolipids. Hence, toxicological screenings against Artemia nauplii, Escherichia coli and Salmonella typhimurium were performed. Toxic effects could only be observed against Artemia, with 48 h-LC50 values for the aqueous and crude extract of methanol of 53.21 mg ml(-1) and 17.81 mg ml(-1), respectively. This study presents the first evidence of a non-nodularin-producing Nodularia isolate in Atlantic salt pan ecosystems and its potential ecotoxicity against Artemia sp. (C) 2009 Elsevier Masson SAS. All rights reserved.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLopes, V. R., Antunes, A., Welker, M., Martins, R. F., & Vasconcelos, V. M. (2010). Morphological, toxicological and molecular characterization of a benthic Nodularia isolated from Atlantic estuarine environments. Research in Microbiology, 161(1), 9-17. https://doi.org/https://doi.org/10.1016/j.resmic.2009.11.001pt_PT
dc.identifier.doi10.1016/j.resmic.2009.11.001pt_PT
dc.identifier.issn1769-7123
dc.identifier.urihttp://hdl.handle.net/10400.22/18587
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0923250809002368?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAtlantic ecosystemspt_PT
dc.subjectEcotoxicitypt_PT
dc.subjectEstuarypt_PT
dc.subjectNodularia sp.pt_PT
dc.subject16S ribosomal RNApt_PT
dc.titleMorphological, toxicological and molecular characterization of a benthic Nodularia isolated from Atlantic estuarine environmentspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage17pt_PT
oaire.citation.startPage9pt_PT
oaire.citation.titleResearch in Microbiologypt_PT
oaire.citation.volume161pt_PT
person.familyNameMartins
person.givenNameRosario
person.identifier.ciencia-idA919-DB40-EF10
person.identifier.orcid0000-0002-9882-4651
person.identifier.scopus-author-id21834368700
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication82a7c349-c88e-450f-adc7-3331d69149cf
relation.isAuthorOfPublication.latestForDiscovery82a7c349-c88e-450f-adc7-3331d69149cf

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