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Autores
Orientador(es)
Resumo(s)
Buses are used by thousands of people in all cities. Passengers are faced with a microenvironment, which may have an impact on their human health. The present study aimed at evaluating and analysing of exposure to biological agents in collective road transport, through the quantification and identification of bacterial and fungal species inside buses. The study carried out in 41 bus trips from January to May 2017. The collection of pure bacterial and pure fungal colonies and their respective isolation was performed by plaque scoring technique, and DNA extraction was performed to identify the species, followed by the PCR technique and sequencing. The 7 identified bacterial species were Staphylococcus argenteus, Bacillus siamensis, Exiguobacterium undae, Psychrobacter faecalis/pulmonis, Exiguobacterium mexicanum, Bacillus paralicheniformis, Exiguobacterium aquaticum. The 6 identified fungal species were Epicoccum nigrum, Clasdoporium haloterans, Cryptococcus diffluens, Cladosporium tenuissimum, Alternaria infectoria and Alternaria tenuissima / Alternaria alternata. Prevention measures such as maintenance of the air-conditioning and ventilation system, use of more efficient cleaning products are intended to ensure safety and comfort for all occupants.
Descrição
Palavras-chave
Fungi Bacterial contamination Air quality Transport
Contexto Educativo
Citação
Santos, J. (2018). Exposição a agentes biológicos: Um estudo de caso em autocarros portugueses. International Symposium on Occupational Safety and Hygiene: Proceedings Book of the SHO2018, 1, 190–192.
Editora
Sociedade Portuguesa de Segurança e Higiene Ocupacionais (SPOSHO)