Loading...
3 results
Search Results
Now showing 1 - 3 of 3
- Integration of the microfluidic and plastic body for the detection of a biomarker associated with Alzheimer diseasePublication . Vinagre, Inês; Chiussi, Stefano; Moreira, Felismina T.C.
- Flexible sensing devices integrating molecularly-imprinted polymers for the detection of 3-nitrotyrosine biomarkerPublication . Martins, Gabriela V.; Riveiro Rodriguez, Antonio; Chiussi, Stefano; Sales, GoretiIn recent years, the development of flexible and wearable devices for healthcare and biomedical applications has become an emerging technological goal, particularly with personalized medicine on the rise. As a response to the increasing demand for in-situ sensing platforms that fulfil some essential requirements like sensitivity, reproducibility and high stability, electrochemical sensors have boosted their way for innovative approaches. So, high-quality flexible sensing strategies are still a demand for local monitoring. Herein, a flexible three-electrode system was fabricated on transparent polymeric sheet substrate through physical deposition of gold as working, counter, and reference electrodes. Along the fabrication process, the electrochemical performance of these electrodes was assessed by means of cyclic voltammetry (CV) while gold adherence to the plastic material was continuously improved. Afterwards, a high-performance molecularly-imprinted sensing film inspired by natural recognition mechanism was assembled through electropolymerization of phenol monomer, in the presence of 3-nitrotyrosine (3-NT), directly on the gold surface. Under the optimized conditions, the flexible (bio)sensor platform was able to detect the presence of 3-NT over the concentration range 10 pg/mL – 1 μg/mL, enabling one of the lower limits of detection found in the literature (1.13 pg/mL or 24.9 pM). The obtained (bio)sensor displayed good reproducibility, stability and selectivity over the chosen interfering substances. Overall, the developed electrochemical device may serve as a flexible, miniaturized, and reliable platform, with potential to be applied in the future as wearable sensing technology.
- Integration of microfluidics and nanoezyme peroxidase for colorimetric detection of neurotransmittersPublication . Sousa, Cristina; Chiussi, Stefano; Moreira, Felismina T.C.The integration of Microfluidics and Nanozyme peroxidase for the Colorimetric detection of Neurotransmitters represents a cutting-edge approach in neurochemical analysis. Microfluidics allows precise manipulation of small fluid volumes, reducing reagent consumption and accelerating reactions while integrating multiple analytical steps on a single chip. Nanozyme peroxidases, mimicking natural peroxidases, provide enhanced stability and catalytic efficiency at a lower cost. The colorimetric detection mechanism signals the presence of neurotransmitters through a visible color change, making it sensitive and easy to interpret for real-time analysis. Detecting neurotransmitters like acetylcholine(ACH) is crucial for understanding and diagnosing various neurological conditions. Acetylcholine plays a key role in physiological processes such as muscle activation, memory function, and synaptic transmission. Abnormal levels are linked to disorders like Alzheimer's disease, myasthenia gravis, and Parkinson's disease. Accurate measurement aids in early diagnosis, monitoring disease progression, and evaluating treatment efficacy. A reliable detection method can advance our understanding of brain function and neurotransmitter dynamics. Real-time monitoring provides insights into how neurotransmitter fluctuations correlate with cognitive functions, behaviors, and responses to stimuli, essential for developing new therapeutic strategies and discovering novel drugs targeting neurotransmitter systems.