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- The effect of high concentrations of vitamin C on a H2O2 - oxidative stress induced yeast modelPublication . Costa, Ana Rita; Cunha, Sofia; Nascimento, Ana Paula; Vieira, Mónica; Prudêncio, CristinaNowadays, the ageing of population is conducting to a rise in the number of individuals with age-related illnesses, in which neurodegenerative diseases (ND) are included. Regardless of many variations in etiology and diverse mechanisms of cell injury, most of ND share high levels of oxidative stress, which have been highly referred not only as an underlying factor, but also as a feature. Thus, an actual challenge is to evaluate the role of antioxidants on oxidative stress states and evaluate the magnitude of possible therapeutic effects of these agents. In the present study, the toxic effect of hydrogen peroxide (H2O2) and the antioxidative function of vitamin C were investigated using Saccharomyces cerevisiae as a model. Results were achieved by exposing the yeast model to different concentrations of H2O2 and vitamin C, itself or combined, and colony forming units counting after 48 h incubation at 30ºC in Yeast Extract Peptone Dextrose agar plates. The results demonstrated that H2O2 exposure decreased yeast cells viability in a dose-dependent manner and that, at an optimal concentration, vitamin C was able to revert its effects. These results improved the understanding of the reversal effect of antioxidant treatment and, therefore, may be helpful on providing insights on a natural antioxidant-based therapy for ND.
- Book of abstracts 4th Meeting on Medicinal Biotechnology (4EBtM)Publication . Costa, Ana Rita; Dias, Ana Rita; Prudêncio, Cristina; Borges, Cristina; Teixeira, Dulce; Almeida, Joana; Vieira, Mónica; Coelho, Pedro; Ferraz, Ricardo; Cunha, SofiaThese meetings have been held once a year since 2015 and have played an important role as a point of contact among several professionals and students in Medicinal Biotechnology. Like the Medicinal Biotechnology field itself, these meetings have also been expanding, not only in the number of participants, but also in their geographical origin. Therefore, it is our goal to act as an interface between students and biotechnology companies/researchers, as well as to increasingly promote networking, projects and collaborations within this field for the upcoming years.
- Book of abstracts of the III Encontro de Biotecnologia Medicinal/ I Iberian Congress on Medicinal BiotechnologyPublication . Costa, Ana Rita; Prudêncio, Cristina; Teixeira, Dulce; Almeida, Joana; Vieira, Mónica; Coelho, Pedro; Ferraz, Ricardo; Cunha, Sofia; Dias, Ana RitaEstes encontros de Biotecnologia Medicinal iniciaram-se em 2015 e sentimos que estamos a crescer, tal como a Biotecnologia Medicinal. Esta área da Biotecnologia tem ganho relevância, quer em empresas, quer a nível académico, criando interfaces entre a biologia molecular, a microbiologia e a química medicinal.