Browsing by Issue Date, starting with "2019-05-17"
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- Study of oxidant and anti-oxidant molecular alterations in an eukaryotic modelPublication . Allen, A.; Cunha, S.; Prudêncio, Cristina; Vieira, MónicaMitochondrial dysfunction and protein aggregation are two phenomena that have been correlated with neurodegenerative diseases and are both interlinked with cellular oxidative stress. Mitochondrial dysfunction is associated with nucleic acid damage and protein aggregation with mistakes in protein folding. However, aggregation has not yet been defined as a cause or consequence of neurodegenerative diseases.
- Optimization and validation of a new cytology stain – CytoPath line®Publication . Teixeira, C.; Fernandes, S.; Silva, R.Cytology plays an important role in the screening and diagnosis of cervical cancer. The correct staining of cervical smear allows the recognition of essential characteristics for the diagnosis of some pathologies. However, given the existence of flaws in the current staining procedures, the development of new staining techniques as well as the optimization of the existing ones is important. Recently, a new staining line - the CytoPath line® is being developed by DiaPath S.p.A. The objective of this work is to evaluate its performance in the staining of gynecological cytology samples.
- Ecotoxicological effect of 1-(2-hydroxyethyl)-3- methylimidazolium chloride and cetylpyridinium chloride towards the microalgae Chlorella vulgarisPublication . Pacheco, Elisabete; Ferraz, Ricardo; Prudêncio, Cristina; Barros, PiedadeIonic liquids (ILs) are salts that are stable over their melting temperature and are made exclusively of ions. ILs have received considerable interest due to their unique properties. The growing interest in ILs predicts an increase of their manufacture and use at industrial scale, which may result in the increased release of these compounds into the environment. In the past years, ILs have been used as a greener alternative to hazardous conventional solvents.
- Antibacterial activity of ionic liquids based on betalactamantibiotics against resistant bacteriaPublication . Dias, Vitorino; Santos, Miguel; Prudêncio, Cristina; Noronha, João P.; Grilo, Inês R.; Sobral, Rita G.; Dias, Ana Rita; Branco, Luís C.; Ferraz, RicardoThe cases of antibiotic resistance are increasing and becoming more and more common, giving rise to a new problem for public health. Therefore, the discovery of new antibiotics is important and necessary.
- Screening of pathogenic variants of the DMD gene in female patients with undetermined muscular dystrophyPublication . Morais, Mara; Gonçalves, Ana; Mota, Sandra; Amorim, ManuelaDystrophinopathy (Duchenne/Becker muscular dystrophy, DMD/BMD), a progressive neuromuscular disease with an X-linked recessive inheritance, is caused by pathogenic variants in the DMD gene, including large deletions (68%), duplications (11%), point variants (20%) and others. Although the majority of females heterozygous for these variants are asymptomatic, about 2.5-7.8% may have some symptom manifestations.
- Book of abstracts 4th Meeting on Medicinal Biotechnology (4EBtM)Publication . Costa, Ana Rita; Dias, Ana Rita; Prudêncio, Cristina; Borges, Cristina; Teixeira, Dulce; Almeida, Joana; Vieira, Mónica; Coelho, Pedro; Ferraz, Ricardo; Cunha, SofiaThese meetings have been held once a year since 2015 and have played an important role as a point of contact among several professionals and students in Medicinal Biotechnology. Like the Medicinal Biotechnology field itself, these meetings have also been expanding, not only in the number of participants, but also in their geographical origin. Therefore, it is our goal to act as an interface between students and biotechnology companies/researchers, as well as to increasingly promote networking, projects and collaborations within this field for the upcoming years.
- Development of a chromatographic method for simultaneous analysis of glutathione formsPublication . Teixeira, Dulce; Coelho, Pedro; Prudêncio, Cristina; Azenha, Manuel; Vieira, MónicaReduced glutathione (GSH)is the most abundant low molecular weight thiol-containing tripeptide (glycine, cysteine, and glutamate) which is synthesized in the cells. GSHplays critical roles in protecting cells from oxidative damage and the toxicity of xenobiotics. Besides, it is also involved in the regulation of intracellular redox homeostasis, which leads to its oxidation into oxidized glutathione (GSSG). Determining the ratio of GSH/GSSG in different biological samples is a major procedure for the evaluation of an individual’s oxidative status and can be a potential biomarker of oxidative stress.
- Effects of glyphosate and cylindrospermopsin at environmental concentrations on growth, photosynthesis and mineral content in lettuce plantsPublication . Sengupta, Soumi; Freitas, Marisa; Pinto, Edgar; Azevedo, Joana; Oliveira, Flávio; Campos, Alexandre; Vasconcelos, VítorGlyphosate is the most widely used herbicide, mainly due to the extensive cultivation of glyphosate resistant plants. The intensification of agriculture has increased water eutrophication and the presence of natural cyanobacterial toxins, such as ylindrospermopsin. Previous studies support the hypothesis that glyphosate and cylindrospermopsin can affect the yield of crop plants, depending on the exposure concentration. Lettuce (Lactuca sativa L.)
- Modulation of oxidative stress with Vitamin E in Sacharomyces cerevisiaePublication . Alves, M.; Cunha, Sofia; Costa, A.; Nascimento, Ana Paula; Vieira da Silva, Manuela; Prudêncio, CristinaNeurodegenerative diseases, such as Parkinson’s Disease or Alzheimer’s Disease, are characterized by the death of a subset of neurons over long periods of time.
- Phytochemical profile, antioxidant activity and cytotoxicity against keratinocytes, fibroblasts and endothelial cells of picoplanktonic marine cyanobacteriaPublication . Morone, Janaína; Lopes, Graciliana; Preto, Marco; Vasconcelos, Vítor; Martins, RosárioCombining the increase demand for natural products in skin care formulations, and the bioactive arsenal of cyanobacteria, we aimed with this study to evaluate the potential of a 70% ethanolic extract of picocyanobacteria strains of the genera Cyanobium and Synechocystis for skin care applications.