A carregar...
2 resultados
Resultados da pesquisa
A mostrar 1 - 2 de 2
- Bioactivity screening of marine cyanobacteria for the isolation of novel compounds for hepatic steatosisPublication . Moreira, Gabriela A.; Rosa, Filipa; Vasconcelos, Vítor; Urbatzka, RalphNonalcoholic fatty liver disease (NAFLD) is a common chronic liver disease and effective therapeutics has been widely study. Cyanobacteria, previously known as blue-green algae, are known to produce many secondary metabolites including toxins, with applications to the pharmaceutical industry. The aim of this study is to investigate the effect of extract from cyanobacteria to reduce lipid accumulation in a model of hepatic steatosis using HepG2 cells in vitro.
- Biotechnological potential of Macroalgae associated ActinomycetotaPublication . Moreira, Gabriela Alves; Girão, Mariana; Eusébio, Nádia; Mendes, Marta V.; Carvalho, Maria de Fátima; Alves Moreira, GabrielaActinomycetota are Gram-positive bacteria widely distributed across diverse environments, from ter-restrial to marine ecosystems, where they may occur as free-living organisms or in association with other organisms. This phylum is well-known for its remark-able biotechnological potential, with several members producing bioactive compounds such as antimicrobial and anticancer agents. However, macroalgae-associ-ated Actinomycetota remain underexplored despite having a metabolic potential comparable to free-living organisms.The main objective of this study was to explore the biosynthetic potential of 15 Actinomycetota isolates obtained from two marine macroalgae from the Por-tuguese coast, Codium tomentosum and Chondrus cris-pus, through the analysis of their genomes.Total DNA was sequenced using Illumina technology and 17 de novo genomes were assembled. Phyloge-nomic analysis identified 5 different genera, Actinoal-loteichus, Cellulosimicrobium, Kocuria, Nocardia and Streptomyces, with 3 putative novel species.Genome mining, using manually curated antiSMASH data, identified 452 biosynthetic gene clusters (BGCs) across 21 metabolite classes. Among the BGCs iden-tified are antimicrobial and anticancer compounds, siderophores and conserved metabolites. More than 60% of these BGCs have none or low similarity to pre-viously known compounds, which can be an indicator of novelty. Compared with Streptomyces, one of the most studied genera, non-Streptomyces genera exhib-ited a higher novelty percentage (ca. 80%).Additionally, BiG-SCAPE and clinker were used to visualize the data and assess the similarity between BGCs for the same compound in different strains.Overall, these results confirm that macroalgae-as-sociated Actinomycetota represent a valuable and underexplored source of new species and biosynthet-ic diversity, supporting further study of this bacterial group.