Loading...
3 results
Search Results
Now showing 1 - 3 of 3
- Flexible 2D and 3D conductive hydrogel platforms for wearable applicationsPublication . Aguiar, Leonor; Pereira, Raquel; Sharma, Sanjiv; Martins, GabrielaHydrogels have risen as exceptionally promising support materials in the development of novel wearable electronic devices. Their remarkable biocompatibility coupled with customizable mechanical features make these biomaterials ideal choices for applications involving direct contact with biological tissues. In this study, a simple and straightforward manufacturing process using bio-sourced polysaccharide chitosan (Chi) was employed for the fabrication of flexible and transparent biopolymeric membranes. Subsequently, this two-dimensional (2D) platform was made conductive, through a one-step process, by utilizing an optimized ratio of chitosan, lactic acid, and silver nanowires (Chi-LaA-AgNWs) dispersion. These electrodes were produced by screen printing technique. Furthermore, a solvent casting technique employing inverse polydimethylsiloxane (PDMS) molds was used to fabricate mechanically stable chitosan microneedles (Chi-MNs). These three dimensional (3D) structures were enriched with a carbon-based ink during the casting of concentrated Chi hydrogels into the mold while utilizing centrifugal forces. The electrochemical properties of the fabricated 2D and 3D conductive platforms were evaluated through cyclic voltammetry (CV). Along this study, the water swelling properties of Chi hydrogels were investigated by incorporating natural crosslinkers and plasticizing compounds like citric acid, glycerol, and sorbitol. Optimization of fabrication, physico-chemical and morphological analysis of the membranes and MNs were also performed. Ultimately, the use of Chi combined with environmentally friendly agents enabled the fabrication of flexible conductive platforms holding good stability, uniformity, and desirable electrical attributes.
- Plastic antibody for the diagnosis of acute myocardial infarctionPublication . Ricardo, Jacinta; Martins, Gabriela; Moreira, Felismina T.C.(Introduction) Novel application Plastic Antibody that responds to a cardiac biomarker, myoglobin (Myo). Imprint stage with electropolymerization of ortho-phenylenediamine (OPD) in the presence of Myo. Template removal from polymeric matrix digested by trypsin. The films acted as biomimetic artificial antibodies and were fabricated on a screen-printed platinium (Pt) electrode (SPE) modified with electroactive Prussian blue nanocubes (PBNCs) to take a step towards disposable sensors for point-of-care applications. The devices showed linear responses to Myo in SWV assays up to 0.01 and 10000 ng/mL.
- Enhanced detection with prussian blue-based nanocubes: a novel electrochemical biosensor for bovine serum albumin analysis: microfluidic integrationPublication . Santos, Andréa dos; Oliveira, Daniela; Martins, Gabriela; Moreira, Felismina T.C.(Introduction) Early diagnosis of biomarkers is crucial for optimizing treatments and increasing survival rates. Direct and cost-effective assays are important for point-of-care (PoC) testing. Bovine serum albumin (BSA) monitoring indicates liver and kidney function and aids in the assessment of diseases such as cirrhosis and chronic kidney disease. An innovative electrochemical biosensor was developed in which a platinum electrode was decorated with manganese-based Prussian blue nanocubes (PB-NC). A molecularly imprinted polymer (MIP) was created on the electrode surface by electropolymerization of phenol in the presence of BSA using cyclic voltammetry (CV). The template was removed with proteinase K and a mixture of methanol and acetic acid. Square wave voltammetry (SWV) characterized the performance of the sensor, which exhibited high sensitivity and allowed detection of BSA at nanomolar levels.