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- Ratiometric determination of CA19-9 using fluorescent carbon dots and yellow-emitting quantum dotsPublication . Rodrigues, E.; Piloto, A.M.L.(Introduction) Quantum dots (QDs) have high fluorescence, chemical stability and sensitivity and optical properties. Traditional QDs probes have a single peak, leading to some problems such as systemic errors due to detection conditions and fluorescence background and systemic instability. Ratiometric methods eliminate random changes in QDs fluorescence intensity and reduces optical fluctuations not caused by the target.