ISEP – CIETI – Pósteres apresentados a eventos científicos
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Browsing ISEP – CIETI – Pósteres apresentados a eventos científicos by Subject "Biomarkers"
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- Detection of CA19-9 using fluorescent polymer imprinted membranesPublication . Xu, Ana; Piloto, Ana Margarida L.(Introduction) The Carbohydrate antigen19-9 (CA19-9) is a biomarker widely used for the diagnosis and monitoring of various types of cancer, especially pancreatic cancer (PC). Molecularly Imprinted Polymers (MIPs) are synthetic materials with recognition sites molecularly designed to bind selectively to a target analyte, providing high specificity and sensitivity. In this work, the use of solid supports such as membranes, was used to imprint the MIPs, allowing the creation of robust and easy-to-manipulate biomimetic sensors for the precise and efficient detection of biomarkers, specifically CA19-9.
- Enhanced detection with prussian blue-based nanocubes: a novel electrochemical biosensor for bovine serum albumin analysis: microfluidic integrationPublication . Santos, Andréa dos; Oliveira, Daniela; Martins, Gabriela; Moreira, Felismina T.C.(Introduction) Early diagnosis of biomarkers is crucial for optimizing treatments and increasing survival rates. Direct and cost-effective assays are important for point-of-care (PoC) testing. Bovine serum albumin (BSA) monitoring indicates liver and kidney function and aids in the assessment of diseases such as cirrhosis and chronic kidney disease. An innovative electrochemical biosensor was developed in which a platinum electrode was decorated with manganese-based Prussian blue nanocubes (PB-NC). A molecularly imprinted polymer (MIP) was created on the electrode surface by electropolymerization of phenol in the presence of BSA using cyclic voltammetry (CV). The template was removed with proteinase K and a mixture of methanol and acetic acid. Square wave voltammetry (SWV) characterized the performance of the sensor, which exhibited high sensitivity and allowed detection of BSA at nanomolar levels.
- Molecularly imprinted polymer for Mucin 4 (MUC4) biomarkerPublication . Piloto, A.M.L.; Cerqueira, N.; Sales, Goreti(Introduction) Molecularly imprinted polymers (MIPs) were synthesized around quantum dots (QDs) to detect the protein biomarker MUC4, which is associated with pancreatic cancer (Kauretal.,2022; Mehrotraetal.,2024). In this approach, MIPs serve as biorecognition elements and are conjugated with cadmium telluride QDs to form the sensing system. The fluorescence of these imprinted polymers is quenched as MUC4 concentrations increase, demonstrating a linear response within the range of 0.12 ng/mL to 16.9 ng/mL in human serum. This range is below the pancreatic cancer diagnostic cut-off values (1.2 to 1.3 ng/mL). The MIPs exhibit good stability and selectivity for MUC4 compared to non-imprinted controls. Additionally, theoretical studies provide valuable insights for the prediction of the functional structure of MIPs, enhancing its selectivity for the target, while reducing time and costs associated with laborious benchtop assays, which may provide an effective tool for tailoring MIPs to other biomarkers for clinical diagnosis.