Percorrer por autor "Silva, Raquel"
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- Liber Domus: development of a prototype RPG for 6th grade mathematics and science learningPublication . Nunes, Eduardo; Gavaia, Bruno; Rodrigues, Rui; Sampaio, Luís; Silva, RaquelRecent studies shed an unfavourable light on existing tools that support at-distance learning in pre-university students. Concerns revolve around motivation, focus, engagement as well as long-term knowledge retention rates. Multiple attempts were made to connect digital games with education, designated by Game-Based Learning, although educational outcomes have been mixed. Possibly due to ad-hoc experimentation, the effects seem related to relationships between specific game mechanisms and structural design. This research project focused on potential effects of a digital 3D role-playing game in learning. A multidisciplinary team built a prototype, named Liber Domus, using game developing techniques and mechanisms that have never been used or explored in the area on this scale. Preliminary results show the game presents students with a more dynamic approach to learning inside the classroom, increasing motivation and fun levels and allowing for a less repetitive revision of subjects, and the flexibility of the tool for both classroom and at-home learning. Further development is ongoing.
- Pigments profile and antioxidant potential of extremophile cyanobacteria isolated from the Mexican Volcanic Lake ChichonalPublication . Silva, Raquel; Gonçalves, Talita; Morone, Janaína; Moreira, Gabriela Alves; Morais, João; Hentschke, Guilherme Scotta; Alvarez-Gutierrez, Peggy Elizabeth; Batista-García, Ramon Alberto; Vasconcelos, Vitor; Lopes, GracilianaCyanobacteria are ancient and prolific microorganisms for which the current knowledge remains limited. The present work constitutes the first report on the presence of the cyanobacteria Tolypothrix sp. LEGE 221228, Nostoc sp. LEGE 221229, Scytonema sp. LEGE 221230 and Drouetiella sp. LEGE 221231 in the volcanic crater lake in Chichonal (Chiapas-Mexico), and in the characterization of their pigments profile and antioxidant potential, through the application of biorefinery approaches. The identification of cyanobacteria species was achieved through advanced morphology-based methods for cyanobacterial taxonomy. The HPLC-PDA analysis of the cyanobacteria acetone extracts revealed β-carotene, echinenone and chlorophyll-a as the main pigments. Drouetiella sp. LEGE 221231 presented the highest β-carotene concentration (63.67 μg/mL), total carotenoids (87.92 μg/mL) and chlorophylls (20.88 μg/mL), closely followed by Tolypothrix sp. LEGE 221228 (with 51.37, 75.14 and 14.88 μg/mL, respectively), this being the first report on the carotenoid and chlorophylls profile of these strains. Aqueous extracts revealed the highest content of phycoerythrin in Tolypothrix sp. LEGE 221228 (50.61 mg/g) and of phycocyanin in Nostoc sp. LEGE 221229 (57.66 mg/g), standing out in superoxide anion radical scavenging, while the acetonic ones were more effective in scavenging nitric oxide radical. For both, Tolypothrix sp. LEGE 221228 was the most effective, presenting the lowest IC50 (53.75 and 57.68 μg/mL, respectively). Overall, the present study enriches the knowledge on biodiversity of cyanobacteria from underexplored environments, enhancing their potential biotechnological applications as producers of added value secondary metabolites with interest in food, pharmaceutical and cosmetic industries.