Browsing by Author "Cunha, S."
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- Análise da relação fenótipo/genótipo HLA no transplante renalPublication . Cunha, S.; Dias, M.; Amorim, Manuela; Condeço, J.A melhor opção terapêutica na maioria dos pacientes em fase final de insuficiência renal é o transplante renal (TR). O estudo da compatibilidade do TR é feito através da caracterização dos antigénios leucocitários humanos (HLA). Neste estudo analisou-se a relação fenótipo/genótipo HLA no TR em 5 pares dador/receptor. Fenotipicamente os pares foram iguais, no entanto, genotipicamente verificou-se a existência de um discordante. Independentemente deste achado, nenhum dos recetores desenvolveu anticorpos específicos contra o enxerto, sugerindo que a relação fenótipo/genótipo HLA não é causa única e indiscutível na rejeição do enxerto.
- Consumption of sweetened beverage in children and its relation with metabolic syndrome: needs assessment and health educationPublication . Teixeira, D.; Cunha, S.; Vieira, Mónica; Dores, Artemisa Rocha; Prudêncio, CristinaThe intake of sugar-sweetened beverage (SSB) has been associated with obesity, type 2 diabetes, cardiovascular diseases and dental caries. Thus, an increased consumption of SSB may potentiate metabolic syndrome (MS). The consumption of SSB has increased amongst children. The promotion of health education at parents/ tutors level may lead to a decrease in SSB intake by children, with an improvement in their quality of life in medium-long term and, consequently, diminished costs for public health.
- Molecular characterization of quinolone resistance mechanisms and extended-spectrum ß-lactamase production in Escherichia coli isolated from dogsPublication . Meireles, D.; Leite-Martins, L.; Bessa, J.; Cunha, S.; Fernandes, Ruben; Matos, A. de; Manaia, C.M.; Costa, P. Martins da; Fernandes, RúbenThe increasing prevalence of antimicrobial resistances is now a worldwide problem. Investigating the mechanisms by which pets harboring resistant strains may receive and/or transfer resistance determinants is essential to better understanding how owners and pets can interact safely. Here, we characterized the genetic determinants conferring resistance to β-lactams and quinolones in 38 multidrug-resistant Escherichia coli isolated from fecal samples of dogs, through PCR and sequencing. The most frequent genotype included the β-lactamase groups TEM (n = 5), and both TEM + CTX-M-1 (n = 5). Within the CTX-M group, we identified the genes CTX-M-32, CTX-M-1, CTX-M-15, CTX-M-55/79, CTX-M-14 and CTX-M-2/44. Thirty isolates resistant to ciprofloxacin presented two mutations in the gyrA gene and one or two mutations in the parC gene. A mutation in gyrA (reported here for the first time), due to a transversion and transition (TCG → GTG) originating a substitution of a serine by a valine in position 83 was also detected. The plasmid-encoded quinolone resistance gene, qnrs1, was detected in three isolates. Dogs can be a reservoir of genetic determinants conferring antimicrobial resistance and thus may play an important role in the spread of antimicrobial resistance to humans and other co-habitant animals.
- Study of oxidant and anti-oxidant molecular alterations in an eukaryotic modelPublication . Allen, A.; Cunha, S.; Prudêncio, Cristina; Vieira, MónicaMitochondrial dysfunction and protein aggregation are two phenomena that have been correlated with neurodegenerative diseases and are both interlinked with cellular oxidative stress. Mitochondrial dysfunction is associated with nucleic acid damage and protein aggregation with mistakes in protein folding. However, aggregation has not yet been defined as a cause or consequence of neurodegenerative diseases.