Browsing by Author "Costa, Paulo S."
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- Low dose irradiation impact on proliferation rate and genetic damage in three-dimensional HepG2 cell culturesPublication . Costa, Pedro; Lemos, Joana; Kocevar, Barbara; Cunha, Lídia; Genésio, Paula; Ponte, Fernanda; Costa, Paulo S.; Sersa, Gregory; Metello, Luís F.Biological effects of low doses of ionizing radiation are not yet consistently studied and documented. In this context, more advanced and complex biological models should be applied to obtain relevant data. Recognizing the need to evolve and develop advanced biological models, in order to try overcome as much as possible known limitations and difficulties of the current monolayer cellular models, as well as the increasing difficulties to use advanced biological models, our group has been working to develop distinct advanced biological alternative models, namely three-dimensional cell cultures.
- Low dose irradiation of three-dimensional cell cultures has an acute effect on proliferation ratePublication . Costa, Pedro; Lemos, Joana; Cunha, Lídia; Genésio, Paula; Ponte, Fernanda; Costa, Paulo S.; Crespo, Paulo; Metello, Luís F.Low doses of ionizing radiation are not yet consistently studied and documented. In such context, more advanced and complex biological models should be applied to obtain relevant data. Recognizing several known limitations and difficulties of the current monolayer cellular models, as well as the increasing difficulties to use advanced biological models, our group has been developing advanced biological alternative models, namely three-dimensional cell cultures. These cellular models (monolayer cellular culture and three-dimensional cell cultures - cultured with encapsulation in 1% alginate and 1,5% agarose coating) were externally irradiated with 100mGy and 1Gy. The consequences of that irradiation were studied in terms of proliferation rate (using MTT assay) and DNA damage (using comet assay), 24h and 72h post-irradiation.
- Modern Radiobiology: Study of Ionizing Radiation Biological Effects using ThreeDimensional Cell CulturesPublication . Costa, Pedro; Lemos, Joana; Cunha, Lídia; Carvalho, António P.; Vasconcelos, Vítor; Genésio, Paula; Ponte, Fernanda; Costa, Paulo S.; Crespo, Paulo; Metello, Luís F.Biological effects of high doses of ionizing radiation are reasonably well studied and documented. Nevertheless, there is a clear lack of information and/or evidence concerning biological effects of Low Doses, namely at medical imaging levels, as those characterizing Nuclear Medicine and Radiology typical environments. Our group has being involved in the in vitro study of these low dose radiobiological effects. Preliminary experimental results with 100mGy external gamma irradiation of HepG2 monolayer cells showed a slight increase in the proliferation rate 24h, 48h and 72h post irradiation. These preliminary results also pointed into the presence of some Bystander effects 72h post irradiation, constituting the starting point and motivation for the need of a more accurate analysis , here presented.