Browsing by Author "Carvalho, Margarida"
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- Development of a novel electrochemical biosensor based on plastic antibodies for detection of STEAP1 biomarker in cancerPublication . Carvalho, Margarida; Rocha, Sandra Moreira; Barroca-Ferreira, Jorge; Maia, Claudio J.; Guillade, Lucía; Correa-Duarte, Miguel A.; Passarinha, Luís A.; Moreira, Felismina T.C.; Gomes, Rui M.STEAP1 is a cell surface protein of the STEAP family whose main function focuses on intercellular communication and cell growth. STEAP1 is considered a promising putative biomarker and a candidate target for prostate cancer treatment. For specific and selective detection of STEAP1, a molecularly imprinted polymers (MIP) was developed on a screen-printed electrode (C-SPE) whose surface was modified with a nanocomposite based on carbon nanotubes decorated with dendritic platinum nanoparticles (CNTs- PAH /Pt). Then, the MIPs were produced on the modified C-SPE by electropolymerization of a mixture of STEAP1 and a monomer (pyrrole-2-carboxylic acid). Then, the protein was removed from the polymeric network by enzymatic treatment with trypsin, which created the specific template cavities for further STEAP1 detection. Electrochemical techniques such as EIS and CV were used to follow the chemical modification steps of C-SPE. The analytical performance of the biosensor was evaluated by SWV in PBS buffer and in lysates of neoplastic prostate cancer cells (LNCaP) extracts. The MIP material showing a linear range from 130 pg/ml to 13 µg/ml. Overall, the biosensor exhibits essential properties such as selectivity, sensitivity and reproducibility for its application in medical and clinical research diagnosis and/or prognosis of prostate cancer.
- Impact of a 10 km race on inflammatory and cardiovascular markers: comparison between trained and untrained recreational adultsPublication . Carvalho, Margarida; Noites, Andreia; Moreira-Gonçalves, Daniel; Ferreira, Rita; Ribeiro, FernandoPrevious studies have found that trained athletes had lower changes in circulating levels of inflammatory biomarkers and cardiovascular stress than untrained athletes, upon prolonged or exhausting exercise. Particularly, recreational runners with less training showed higher risk of cardiac injury and dysfunction after a marathon. Presently, we are observing a steadily growing number of young and older adults engaging in running events without having a professional orientation or training, emphasizing the need to assess biochemical markers that allow the evaluation of the acute changes imposed in these recreational athletes. To compare the immediate and 24-hour effects of a 10-km run on inflammatory and cardiovascular biomarkers between recreational athletes, with and without specific running training.
- Irreversible temperature indicator based cellulose membranes conjugated with leuco-dye pigmentPublication . Carvalho, Margarida; Tavares, Ana P. M.; Marques. Maria A.; Alves, Joaquim J.; Figueira, Joana; Morais, Maria; Pinto, Joana V.; Moreira, Felismina T. C.This research focuses on the development of thermochromic membranes made of cellulose acetate (CA) for temperature monitoring of sensitive food products. Two dual TC membranes developed for the control of different temperature ranges were formulated using a three-component system: a leuco-dye membrane (crystal violet lactone, CVL) integrated with an acidic membrane containing the color developer (salicylic acid) and the acidic solvent with different melting points (decanoic acid, DA, or methacrylic acid, MA). The CVL membrane, together with the DA membrane, showed an irreversible color change when exposed to 35°C, which was facilitated by the melting of DA. The CVL membrane also underwent an irreversible color change when exposed to 15°C together with the MA membrane. The membranes were characterized in detail using scanning electron microscopy. The evaluation of color changes, reproducibility, specificity, and stability ensured the practical suitability of these membranes. Overall, this innovative approach has proven to be a reproducible, sustainable, cost-effective method to produce irreversible colorimetric temperature sensors. These sensors have significant potential for applications in the food and pharmaceutical industries and offer a promising way to improve product safety and quality.