Percorrer por autor "Barth, Ortrud Monika"
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- A multivariate approach based on physicochemical parameters and biological potential for the botanical and geographical discrimination of Brazilian bee pollenPublication . De-Melo, Adriane Alexandre Machado; Estevinho, Leticia M.; Moreira, Manuela M.; Delerue-Matos, Cristina; Freitas, Alex da Silva de; Barth, Ortrud Monika; Almeida-Muradian, Ligia Bicudo deFifty six Brazilian bee pollen samples were characterised, and their botanical and geographical origins were studied. The samples were also classified based on physicochemical and biological properties. The characterisation was done using Soxhlet extraction and micro-Kjeldahl methods, ashing at 550ºC, transmittance, microscopy, spectrophotometry, inductively coupled plasma optical emission spectrometry and high-performance liquid chromatography. As expected, the values of proteins (7.9–32.2 g/100 g), lipids (3.2–13.5 g/100 g), ash (1.9–3.6 g/100 g), carbohydrates (54.9–82.8 g/100 g), K (3.4–9.8 g/Kg), Ca (0.9–4.1 g/Kg), Mg (0.6–2.4 g/Kg), Fe (46–1180 mg/Kg), Na (20–374 mg/Kg), Mn (25–215 mg/Kg), Zn (30–101 mg/Kg), Cu (7.4–19.7 mg/Kg), total phenolics (6.5–29.2 mg GAE/g) and flavonoids (0.3–17.5 mg QE/g) as well as the phenolic profiles varied among the samples. In general, the products had high antioxidant capacity (ORAC: 133–563 mmol TE/g; DPPH: 9.4–155 mmol TE/g) and the antimicrobial capacity varied according to the sample and microorganism tested. The instrumental colour parameters could be used as indicators of some minerals. Based on multivariate approaches, bee pollen from Southern Brazil seemed to have a higher biological potential, whereas products from the Northeast region had higher calcium and magnesium levels. Similarities were identified in bee pollen containing C. nucifera or Brassica, and monofloral bee pollen seemed to have higher mineral content than heterofloral bee pollen.
- Phenolic profile by HPLC-MS, biological potential, and nutritional value of a promising food: Monofloral bee pollenPublication . De-Melo, Adriane Alexandre Machado; Estevinho, Leticia M.; Moreira, Manuela M.; Delerue-Matos, Cristina; Freitas, Alex da Silva de; Barth, Ortrud Monika; Almeida-Muradian, Ligia Bicudo deThe objective of this study was to determine physicochemical and phenolic profiles as well the antioxidant and antimicrobial capacities of monofloral bee pollen samples from Brazil. Traditional methods were used. The phenolic profile was determined by high‐performance liquid chromatography‐mass spectrometry (HPLC‐MS). The protein (10.6–33.9 g/100g), lipids (3.2–8.3 g/100g), ashes (2.6–3.8 g/100g), total phenolic (5.6–29.7 mg GAE/g), and total flavonoid (0.3–19.0 mg QE/g) values were variable, even between products with the same botanical origin. The minerals analyzed were found in amounts varying between 10 mg/kg and 9.1 g/kg. Each product presented specific color parameters. Myrcia bee pollen presented the greater antioxidant capacity, and Mimosa scabrella bee pollen from Santa Catarina state was the most efficient in inhibiting bacteria and yeasts. Among the compounds identified by HPLC‐MS, flavonoid 3‐O‐glycosides predominated. The physicochemical and phenolic profiles of each sample were distinct, and there was no pattern between monofloral products of the same pollen type.