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ESS - CQB - Ciências Químicas e das Biomoléculas

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http://hdl.handle.net/10400.22/229

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Percorrer ESS - CQB - Ciências Químicas e das Biomoléculas por autor "Abreu, M. J."

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  • Development of a sustainable biosensor to detect respiratory infectious diseases
    Publication . Nascimento, Ana Margarida; Abreu, Cristina; Silva, M.; Leite, B.; Rouly, S.; Abreu, M. J.; Ferraz, Ricardo; Costa, M.; Prudêncio, Cristina; Parente, E.; Vieira, Mónica; Ferraz, Ricardo; Vieira, Mónica; Prudêncio, Cristina
    The Covid-19 pandemic brought the need to use social masks to prevent the spread of the SARS-CoV-2 virus. However, no reliable and fast method were yet established to detect viral particles and to improve the protective ability of social masks. Through color changes, colorimetric biosensors can be used as a rapid and easily approach to detect virus. Gold nanoparticles (AuNP) are known to have excellent optical properties and huge research potential. The new SARS-CoV-2 has the ability of entering human body cells, namely through a second pathway of entry – the sialic acid (SA) receptor. In order to respond to the emergency and to contribute to the diminishing of the spread of SAR-CoV-2, we developed a colorimetric biosensor based on the functionalization AuNP by sialic acid (SA) (SA-AuNP), as a new and effective textile coating layer, to provide a direct indication of the protective capacity of social masks. To do that, AuNPs (10 nm) were functionalized with SA (SA-AuNP), in three different concentrations (50-50, 30-70 and 20-80, respectively) to select the optimal concentration for respiratory virus detection. Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscope with a Transmission Detector (STEM) analyses confirmed SA-AuNPs binding. FTIR results showed a well-established bond, through matches of peaks of SA-AuNPs. Bindings between the compounds were more evident in 50-50 concentration of SA-AuNP. In the 30-70 SA-AuNP the STEM images show some superposition of the nanoparticles and not so evident binding, as in the 20-80 concentration. Still, between these last two concentrations, the 30-70 is the one that shows the best results since it is visible some circular points larger than the others. To achieve the goal, the concentrations 30-70 and 50-50 of SA-AuNP were impregnated (Textile Foulard) in two substrates of different compositions, a cellulosic and a synthetic one. However, in this technique there are many parameters, such as drying time and temperature, which were varied to understand which the best procedure was to obtain the biosensor.
    2022-12-01Artigo de investigação Acesso aberto Ver mais