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Advanced botanical authentication of honey: Using an ultrasensitive electrochemical genosensor and RT-qPCR for the detection of Castanea sativa

2026-03-28Preprint Acesso aberto
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dc.contributor.authorMorais, Stephanie
dc.contributor.authorPereira, Eduarda
dc.contributor.authorFerreira, Mariana
dc.contributor.authorSantos, Marlene
dc.contributor.authorSoares, Sónia
dc.contributor.authorTexeira, Ana L.
dc.contributor.authorDomingues, Valentina
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorBarroso, M. Fátima
dc.contributor.authorSantos, Marlene
dc.date.accessioned2026-07-06T13:50:38Z
dc.date.available2026-07-06T13:50:38Z
dc.date.issued2026-03-28
dc.description.abstractFood fraud is a reoccurring issue for the food industry, with significant public health and economic implications. Honey, a natural ingredient prized for its sweetness and inherent nutritional profile and health benefits, is one of the most frequently adulterated foods found in the international market. This fraudulent act not only damages the reputation of the honey industry but also presents a hazard to the consumers’ health. So, in this study, a disposable electrochemical genosensor was developed to detect Castanea sativa (chestnut tree) DNA in commercial honey samples. For this, a 103 bp C. sativa specific DNA-target oligonucleotide and its complementary probe were selected and designed. The genosensor methodology implied a sandwich hybridization format, for which the complementary sequence was cut into a 22 bp thiolated DNAcapture probe and an 81 bp fluorescein isothiocyanate-labelled DNA-signaling probe. Using chronoamperometric measurements, the enzymatic amplification of the electrochemical signal was obtained in a 0.03 to 1.00 nM concentration range, with a LOD and LOQ of 3.01 and 10.04 pM, respectively. The developed genosensor was able to detect the presence of the chestnut DNA in real chestnut plants and commercial honey samples. These results were then validated real-time quantitative PCR (RT-qPCR). In fact, conventional PCR coupled with gel electrophorese was not able to detect the presence of heather in honey. Therefore, electrochemical genosensors are a promising and cost-effective analytical tool to authenticate the botanical origin of honey, guaranteeing its safety, quality and authenticity.eng
dc.description.sponsorshipCOMPETE2030-FEDER-00699000
dc.identifier.citationMorais, S., Pereira, E., Ferreira, M., Santos, M. P., Soares, S., Teixeira, A. L., Domingues, V. F., Delerue-Matos, C., & Barroso, M. F. (2026). Advanced botanical authentication of honey: Using an ultrasensitive electrochemical genosensor and RT-qPCR for the detection of Castanea sativa (SSRN Scholarly Paper N. 6479780). Social Science Research Network. https://doi.org/10.2139/ssrn.6479780
dc.identifier.doi10.2139/ssrn.6479780
dc.identifier.urihttp://hdl.handle.net/10400.22/32552
dc.language.isoeng
dc.peerreviewedno
dc.publisherElsevier
dc.relationUID/50006/2025
dc.relation.hasversionhttps://papers.ssrn.com/sol3/papers.cfm?abstract_id=6479780
dc.rights.uriN/A
dc.subjectCastanea sativa
dc.subjectFood fraud
dc.subjectElectrochemical genosensor
dc.subjectHoney authentication
dc.subjectRT-qPCR
dc.titleAdvanced botanical authentication of honey: Using an ultrasensitive electrochemical genosensor and RT-qPCR for the detection of Castanea sativaeng
dc.typepreprint
dspace.entity.typePublication
oaire.versionhttp://purl.org/coar/version/c_71e4c1898caa6e32
person.familyNameSantos
person.givenNameMarlene
person.identifier1508370
person.identifier.ciencia-id8311-B967-31C4
person.identifier.orcid0000-0001-5020-5942
person.identifier.scopus-author-id57110502000
relation.isAuthorOfPublication8ce9ee39-a4c6-46ae-99e2-49397b550f1b
relation.isAuthorOfPublication.latestForDiscovery8ce9ee39-a4c6-46ae-99e2-49397b550f1b

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