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The optical dispersion and water content of human liver were experimentally studied to estimate the optical dispersions of tissue scatterers and dry matter. Using temporal measurements of collimated transmittance [Tcðt Þ] of liver samples under treatment at different glycerol concentrations, free water and diffusion coefficient (Dgl) of glycerol in liver were found as 60.0% and 8.2 × 10−7 cm2∕s, respectively. Bound water was
calculated as the difference between the reported total water of 74.5% and found free water. The optical
dispersion of liver was calculated from the measurements of refractive index (RI) of tissue samples made
for different wavelengths between 400 and 1000 nm. Using liver and water optical dispersions at 20°C and
the free and total water, the dispersions for liver scatterers and dry matter were calculated. The estimated
dispersions present a decreasing behavior with wavelength. The dry matter dispersion shows higher RI values than liver scatterers, as expected. Considering 600 nm, dry matter has an RI of 1.508, whereas scatterers have an RI of 1.444. These dispersions are useful to characterize the RI matching mechanism in optical clearing treatments, provided that [Tcðt Þ] and thickness measurements are performed during treatment. The knowledge of Dgl is also important for living tissue cryoprotection applications.
Descrição
Palavras-chave
Human liver tissue Refractive index Free and bound water Optical clearing Diffusion coefficient Cryoprotection
Contexto Educativo
Citação
Editora
Society of Photo-optical Instrumentation Engineers