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Involvement of the Iron-Regulated Loci hts and fhuC in Biofilm Formation and Survival of Staphylococcus epidermidis within the Host

2022-01-12Journal article Open access
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dc.contributor.authorOliveira, Fernando
dc.contributor.authorLima, Tânia
dc.contributor.authorCorreia, Alexandra
dc.contributor.authorSilva, Ana Margarida
dc.contributor.authorSoares, Cristina
dc.contributor.authorMorais, Simone
dc.contributor.authorWeißelberg, Samira
dc.contributor.authorVilanova, Manuel
dc.contributor.authorRohde, Holger
dc.contributor.authorCerca, Nuno
dc.date.accessioned2023-01-30T11:26:30Z
dc.date.available2023-01-30T11:26:30Z
dc.date.issued2022-01-12
dc.description.abstractStaphylococcus epidermidis is a major nosocomial pathogen with a remarkable ability to persist on indwelling medical devices through biofilm formation. Nevertheless, it remains intriguing how this process is efficiently achieved under the host’s harsh conditions, where the availability of nutrients, such as essential metals, is scarce. Following our previous identification of two iron-regulated loci putatively involved in iron transport, hts and fhuC, we assessed here their individual contribution to both bacterial physiology and interaction with host immune cells. Single deletions of the hts and fhuC loci led to marked changes in the cell iron content, which were partly detrimental for planktonic growth and strongly affected biofilm formation under iron-restricted conditions. Deletion of each of these two loci did not lead to major changes in S. epidermidis survival within human macrophages or in an ex vivo human blood model of bloodstream infection. However, the lack of either hts or fhuC loci significantly impaired bacterial survival in vivo in a murine model of bacteremia. Collectively, this study establishes, for the first time, the pivotal role of the iron-regulated loci hts and fhuC in S. epidermidis biofilm formation and survival within the host, providing relevant information for the development of new targeted therapeutics against this pathogenpt_PT
dc.description.sponsorshipWe acknowledge the assistance of Nurse Filomena and thank all the blood donors. N.C. and M.V. acknowledge the support by the Portuguese Foundation for Science and Technology (FCT) through the funded project PTDC/BIAMOL/29553/2017, under the scope of COMPETE2020 (POCI-01-0145-FEDER-029553). N.C. acknowledges the strategic funding of UID/BIO/04469/2019 unit by FCT. M.V. acknowledge the support of the i3S Scientific Platform HEMS, member of the Portuguese Platform of BioImaging (PPBI) (PPBIPOCI-01-0145-FEDER-022122), funded by FCT. H.R. acknowledges support from the German Research Council (DFG Ro 2413/4-1), the Damp Foundation (2013-19), and the Joachim Herz Foundation. F.O. was supported by an individual Ph.D. scholarship (SFRH/BD/101399/2014). F.O., M.V., H.R., and N.C. designed research; F.O., T.L., A.M.S., and C.S. performed research; S.M. contributed new reagents/analytic tools; F.O., T.L., A.C., C.S., S.M., and S.W. analyzed data; M.V., H.R., and N.C. supervised research; and F.O., M.V., H.R., and N.C. wrote the paper. All authors read and approved the final version of the manuscriptpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1128/spectrum.02168-21pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/21992
dc.language.isoengpt_PT
dc.publisherASMpt_PT
dc.relationPTDC/BIAMOL/29553/2017pt_PT
dc.relationCentre of Biological Engineering - University of Minho
dc.relationThe role of iron uptake in Staphylococcus epidermidis biofilm formation and virulence
dc.relation.publisherversionhttps://journals.asm.org/doi/10.1128/spectrum.02168-21pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectBiofilmspt_PT
dc.subjectInnate immunitypt_PT
dc.subjectIronpt_PT
dc.titleInvolvement of the Iron-Regulated Loci hts and fhuC in Biofilm Formation and Survival of Staphylococcus epidermidis within the Hostpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre of Biological Engineering - University of Minho
oaire.awardTitleThe role of iron uptake in Staphylococcus epidermidis biofilm formation and virulence
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FBIO%2F04469%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F101399%2F2014/PT
oaire.citation.issue1pt_PT
oaire.citation.titleMicrobiology Spectrumpt_PT
oaire.citation.volume10pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPOR_NORTE
person.familyNameDias Soares
person.familyNameMorais
person.givenNameCristina Maria
person.givenNameSimone
person.identifier1598822
person.identifier.ciencia-id5016-4F99-C69D
person.identifier.ciencia-idB111-BBFB-F200
person.identifier.orcid0000-0003-0924-9681
person.identifier.orcid0000-0001-6433-5801
person.identifier.scopus-author-id7007053747
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication72b9814d-0e56-42e0-a1d7-93cb47fb9ae0
relation.isAuthorOfPublication7a9c7d63-a6c7-4159-b12d-b049b8c5c8f9
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