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Tissue optical clearing mechanisms

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dc.contributor.authorYu, T.
dc.contributor.authorZhu, Dan
dc.contributor.authorGenina, Elina A.
dc.contributor.authorOliveira, Luís
dc.contributor.authorBashkatov, A.N
dc.contributor.authorTuchin, V.V
dc.date.accessioned2022-02-24T11:56:34Z
dc.date.embargo2031
dc.date.issued2022
dc.description.abstractThe high scattering and absorption of opaque tissues limit the penetration of light into deep tissues, limiting the imaging depth of various optical imaging techniques. The proposed tissue optical clearing technique provides an innovative way to perform deep-tissue imaging. Recently, various optical clearing methods have been developed. They result in tissue clearing with similar physical principles but different chemical approaches. This chapter will introduce the mechanisms of the current tissue optical clearing methods, ranging from fundamental physical to chemical perspectives, including the main physical principle, refractive index matching; and chemical approaches such as dissociation of collagen, dehydration, delipidation, decalcification, and hyperhydration to reduce scattering, as well as the use of decolorization to reduce absorption.pt_PT
dc.description.sponsorshipT.Y. and D.Z. were supported by the National Key Research and Development Program of China grant number 2017YFA0700501, and NSFC grant number 81171736. E.A.G, A.N.B., and V.V.T were supported by RFBR grant number 18-52-16025. L. O. was supported by the Portuguese Science Foundation grant number FCT-UIDB/04730/2020. The authors are thankful to the staff of the Portuguese Oncology Institute of Porto (IPO-Porto), Portugal, for providing the human colorectal specimens used in the research presented in this chapter. The authors also appreciate the histological photograph of the human colorectal wall, which was supplied by Dr Sónia Carvalho from IPO-Porto.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationYu T., Zhu D., Oliveira L., Genina E.A., Bashkatov A.N., Tuchin V.V. (2021) Tissue optical clearing mechanisms. In: Tuchin V.V., Zhu D., Genina E.A. (eds). Handbook of Tissue Clearing: New Prospects in Optical Imaging. (Ch. 1, pp. 3-29). CRC Press, London 2021. ISBN 9780367895099, ISBN 9781003025252 (ebook), Chapter DOI: 10.1201/9781003025252.pt_PT
dc.identifier.doi10.1201/9781003025252pt_PT
dc.identifier.eissn9781003025252
dc.identifier.issn9780367895099
dc.identifier.urihttp://hdl.handle.net/10400.22/20053
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherCRC Presspt_PT
dc.relation2017YFA0700501pt_PT
dc.relation81171736pt_PT
dc.relation18-52-16025pt_PT
dc.relationFCT-UIDB/04730/2020pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectOptical clearingpt_PT
dc.subjectDeep-tissue imagingpt_PT
dc.titleTissue optical clearing mechanismspt_PT
dc.typebook part
dspace.entity.typePublication
oaire.citation.conferencePlaceLondonpt_PT
oaire.citation.endPage29pt_PT
oaire.citation.startPage3pt_PT
oaire.citation.titleHandbook of Tissue Optical Clearingpt_PT
person.familyNameOliveira
person.givenNameLuís
person.identifier414820
person.identifier.ciencia-idC312-F342-BD23
person.identifier.orcid0000-0003-0667-3428
person.identifier.ridB-7198-2017
person.identifier.scopus-author-id55853723900
rcaap.rightsclosedAccesspt_PT
rcaap.typebookPartpt_PT
relation.isAuthorOfPublication4e2de4d7-142a-400f-8dbf-cd0ff9551093
relation.isAuthorOfPublication.latestForDiscovery4e2de4d7-142a-400f-8dbf-cd0ff9551093

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