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Novel and simple electrochemical biosensor monitoring attomolar levels of miRNA-155 in breast cancer

2016Journal article Restricted access
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dc.contributor.authorCardoso, Ana Rita
dc.contributor.authorMoreira, Felismina
dc.contributor.authorFernandes, Rúben
dc.contributor.authorSales, Goreti
dc.date.accessioned2017-07-13T11:52:39Z
dc.date.embargo2117
dc.date.issued2016
dc.description.abstractThis work, describes for the first time, a simple biosensing design to yield an ultrasensitive electro-chemical biosensor for a cancer biomarker detection, miRNA-155, with linear response down to the attomolar range. MiRNA-155 was selected for being overexpressed in breast cancer. The biosensor was assembled in two stages: (1) the immobilization of the anti-miRNA-155 that was thiol modified on an Au-screen printed electrode (Au-SPE), followed by (2) blocking the areas of non-specific binding with mercaptosuccinic acid. Atomic force microscopy (AFM) and electrochemical tech-niques including cyclic voltammetry (CV), impedance spectroscopy (EIS) and square wave voltammetry (SWV) confirmed the surface modification of these devices and their ability to hybridize successfully and stably with miRNA-155. The final biosensor provided a sensitive detection of miRNA-155 from 10 aM to 1.0 nM with a low detection limit (LOD) of 5.7 aM in real human serum samples. Good results were obtained in terms of selectivity towards breast cancer antigen CA-15.3 and bovine serum albumin (BSA). Raw fluid extracts from cell-lines of melanoma did not affect the biosensor response (no significant change of the blank), while raw extracts from breast cancer yielded a positive signal against miRNA-155. This simple and sensitive strategy is a promising alternative for simultaneous quantitative analysis of multiple miRNA in physiological fluids for biomedical research and point-of-care (POC) diagnosis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.bios.2016.02.035pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/10049
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.ispartofseriesBiosensors and Bioelectronics;Vol. 80
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0956566316301427pt_PT
dc.subjectBreast cancerpt_PT
dc.subjectAnti-miRNA-155pt_PT
dc.subjectmiRNA-155pt_PT
dc.subjectBiosensorspt_PT
dc.subjectEletrochemistrypt_PT
dc.titleNovel and simple electrochemical biosensor monitoring attomolar levels of miRNA-155 in breast cancerpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleBiosensors and Bioelectronicspt_PT
person.familyNameCardoso
person.familyNameMoreira
person.familyNameFernandes
person.familyNameFerreira Sales
person.givenNameAna Rita
person.givenNameFelismina
person.givenNameRúben
person.givenNameMaria Goreti
person.identifier1589429
person.identifier635792
person.identifier826970
person.identifier.ciencia-idF717-044B-1E8D
person.identifier.ciencia-idC917-6A46-A270
person.identifier.ciencia-id0D1F-4090-E82A
person.identifier.ciencia-idE31F-1630-42BB
person.identifier.orcid0000-0002-8641-0865
person.identifier.orcid0000-0003-4237-8952
person.identifier.orcid0000-0001-8933-3984
person.identifier.orcid0000-0001-9936-7336
person.identifier.ridK-9199-2013
person.identifier.scopus-author-id57125522400
person.identifier.scopus-author-id23486193000
person.identifier.scopus-author-id57640135700
person.identifier.scopus-author-id7005174717
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationeeb95820-2553-4ad8-8d44-501ffd2c76ff
relation.isAuthorOfPublication9c13153d-a73a-49ff-b7f6-c9733bc6725e
relation.isAuthorOfPublication9e6c397c-62d8-4a40-8ec7-ad05ae0ebcc4
relation.isAuthorOfPublicationb6d3a2d0-608a-4a92-834b-8d7b29069fef
relation.isAuthorOfPublication.latestForDiscovery9e6c397c-62d8-4a40-8ec7-ad05ae0ebcc4

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