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Candida glabrata susceptibility to antifungals and phagocytosis is modulated by acetate

2015Journal article Open access
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dc.contributor.authorMota, Sandra
dc.contributor.authorAlves, Rosana
dc.contributor.authorCarneiro, Catarina
dc.contributor.authorSilva, Sónia
dc.contributor.authorBrown, Alistair J.
dc.contributor.authorIstel, Fabian
dc.contributor.authorKuchler, Karl
dc.contributor.authorSampaio, Paula
dc.contributor.authorCasal, Margarida
dc.contributor.authorHenriques, Mariana
dc.contributor.authorPaiva, Sandra
dc.date.accessioned2016-01-21T12:57:47Z
dc.date.available2016-01-21T12:57:47Z
dc.date.issued2015
dc.description.abstractCandida glabrata is considered a major opportunistic fungal pathogen of humans. The capacity of this yeast species to cause infections is dependent on the ability to grow within the human host environment and to assimilate the carbon sources available. Previous studies have suggested that C. albicans can encounter glucose-poor microenvironments during infection and that the ability to use alternative non-fermentable carbon sources, such as carboxylic acids, contributes to the virulence of this fungus. Transcriptional studies on C. glabrata cells identified a similar response, upon nutrient deprivation. In this work, we aimed at analyzing biofilm formation, antifungal drug resistance, and phagocytosis of C. glabrata cells grown in the presence of acetic acid as an alternative carbon source. C. glabrata planktonic cells grown in media containing acetic acid were more susceptible to fluconazole and were better phagocytosed and killed by macrophages than when compared to media lacking acetic acid. Growth in acetic acid also affected the ability of C. glabrata to form biofilms. The genes ADY2a, ADY2b, FPS1, FPS2, and ATO3, encoding putative carboxylate transporters, were upregulated in C. glabrata planktonic and biofilm cells in the presence of acetic acid. Phagocytosis assays with fps1 and ady2a mutant strains suggested a potential role of FPS1 and ADY2a in the phagocytosis process. These results highlight how acidic pH niches, associated with the presence of acetic acid, can impact in the treatment of C. glabrata infections, in particular in vaginal candidiasis.pt_PT
dc.identifier.citationMota, S., Alves, R., Carneiro, C., Silva, S., Brown, A. J., Istel, F., Kuchler, K., Sampaio, P., Casal, M., Henriques, M., & Paiva, S. (2015). Candida glabrata susceptibility to antifungals and phagocytosis is modulated by acetate. Frontiers in Microbiology, 6(article 919), 1–12. https://doi.org/10.3389/fmicb.2015.00919
dc.identifier.doi10.3389/fmicb.2015.00919pt_PT
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/10400.22/7443
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontierspt_PT
dc.relation.publisherversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4560035/pt_PT
dc.subjectCandida glabratapt_PT
dc.subjectAcetatept_PT
dc.subjectTransporterspt_PT
dc.subjectPhagocytosispt_PT
dc.subjectAntifungal drug resistancept_PT
dc.subjectFluconazolept_PT
dc.subjectCandidiasispt_PT
dc.titleCandida glabrata susceptibility to antifungals and phagocytosis is modulated by acetatept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage12pt_PT
oaire.citation.issuearticle 919pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleFrontiers in Microbiologypt_PT
oaire.citation.volume6pt_PT
person.familyNameMota
person.givenNameSandra
person.identifier.ciencia-id0A19-207B-6C63
person.identifier.orcid0000-0002-2803-7230
person.identifier.scopus-author-id6603695681
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationf73a5c8e-1621-435f-a34f-c3cc83924875
relation.isAuthorOfPublication.latestForDiscoveryf73a5c8e-1621-435f-a34f-c3cc83924875

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