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Chemical Characterization and In Vitro Bioactivity of Apple Bark Extracts Obtained by Subcritical Water

2021-05-24Journal article Restricted access
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dc.contributor.authorŠvarc-Gajić, Jaroslava
dc.contributor.authorCerdà, Víctor
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorMašković, Pavle
dc.contributor.authorClavijo, Sabrina
dc.contributor.authorSuarez, Ruth
dc.contributor.authorCvetanović, Aleksandra
dc.contributor.authorRamalhosa, Maria João
dc.contributor.authorBarroso, M. Fátima
dc.contributor.authorMoreira, Manuela
dc.contributor.authorMorais, Simone
dc.contributor.authorWithouck, Hannes
dc.contributor.authorBoeykens, Annick
dc.date.accessioned2021-07-14T14:50:30Z
dc.date.embargo2100
dc.date.issued2021-05-24
dc.date.updated2021-07-14T14:20:46Z
dc.description.abstractRecently, the value of wood residues has received increasing attention due to the presence of pharmacologically active compounds. In the present study, apple bark was treated with subcritical water (SWE) with the aim of bioactivity determination and chemical analysis by GC–MS applying two different sample preparation protocols and by HPLC. Extracts were screened for their total antioxidant capacity by means of total phenolic (TPC) and flavonoid contents (TFC), ferric reducing antioxidant power (FRAP) and DPPH-radical scavenging activity (DPPH-RSA). Anti-microbial and anti-proliferative activities of the apple bark subcritical water extracts were also examined. Different lignin and cellulose degradation products were identified by GC–MS, as well as naturally present compounds. Identified compounds encompassed organic and fatty acids, alcohols, sugars, phenol, benzaldehyde, cinnamic acid, cinnamaldehyde and azulene derivatives, as well as other organic compounds. Phenolic profiles of apple bark extracts defined by HPLC–PDA analysis revealed that the main contributors were gallic acid (9.4 ± 0.5 mg/g DW) and catechin (8.3 ± 0.4 mg/g DW). The diversity of the extracts composition supports the fact that this medium is able to extract compounds of different polarities, simultaneously acting as catalyst and reactant. The characterized apple bark extracts demonstrated good antioxidant (TPC = 31.47 ± 1.86 mg GAE/g; TFC = 17.40 ± 1.89 mg EE/g; FRAP = 22.45 ± 2.22 mg AAE/g; DPPH-RSA = 22.57 ± 2.24 mg Trolox E/g DW), antimicrobial (MIC = 19.53–156.25 μg/mL) and anti-proliferative activity (IC50 = 19.88–47.44 μg/mL). The findings of this study showed that SWE can be used as an environmentally friendly and safe technique for the valorization of apple tree wood residues, applicable in food and pharmaceutical industries.pt_PT
dc.description.sponsorshipThe authors are grateful to the Serbian Ministry of Education, Science and Technological Development (451-03-68/2020-14/200134), Provincial Secretariat for High Education and Scientific Research AP Vojvodina, Serbia (Project No. 142-451-3240/2020-03), Provincial Secretariat for Science and Technological Development and Portuguese Ministry of Science (FCT/Servia 2015/2016 proc. 441.00 SERVIA). Financial support from the Ministerio de Economía y Competitividad (Spanish Government) through the Grant CTQ2016-77155-R is also gratefully acknowledged. R. Suárez thanks to the Conselleria d’Educació, Cultura i Universitats from the Government of the Balearic Islands for a PhD stipend co-financed by Fondo Social Europeo (FPI/1444/2012). S. Clavijo acknowledges to Torres Quevedo Program of the Ministerio de Economía y Competitividad (MINECO) co-financed by European Funds, for the financial support through the PTQ 15-08038. This work was also supported by UIDB/50006/2020 and UIDP/50006/2020 by the Fundação para a Ciência e a Tecnologia (FCT)/Ministério da Ciência, Tecnologia e Ensino Superior (MCTES) through national funds. Manuela M. Moreira (project CEECIND/02702/2017) is grateful for the financial support financed by national funds through FCT and to REQUIMTE/LAQV. Manuela M. Moreira (SFRH/BPD/97049/2013) and M. F. Barroso (SFRH/BPD/78845/2011) are grateful for the postdoctoral fellowships financed by POPH-QREN—Tipologia 4.1—Formação Avançada, subsidized by Fundo Social Europeu and Ministério da Ciência, Tecnologia e Ensino Superior. The financial support from Fundação para a Ciência e Tecnologia/ Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MEC) through national funds and co-financed by European Regional Development Fund (FEDER), under the Partnership Agreement PT2020 through the project UID/ QUI/50006/2013—POCI/01/0145/FERDER/007265 is also acknowledged. Annick Boeykens wishes to acknowledge Odisee for funding by means of a PWO grant (Flemish Government; Belgium).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s12649-021-01477-zpt_PT
dc.identifier.issn1877-2641
dc.identifier.issn1877-265X
dc.identifier.slugcv-prod-2551719
dc.identifier.urihttp://hdl.handle.net/10400.22/18118
dc.language.isoengpt_PT
dc.publisherSpringerpt_PT
dc.relationFCT/Servia 2015/2016 proc. 441.00 SERVIApt_PT
dc.relationFPI/1444/2012pt_PT
dc.relation451-03-68/2020-14/200134pt_PT
dc.relation142-451-3240/2020-03pt_PT
dc.relationCTQ2016-77155-Rpt_PT
dc.relationPTQ 15-08038pt_PT
dc.relationCEECIND/02702/2017pt_PT
dc.relationVALORIZAÇÃO DE RESÍDUOS DA INDÚSTRIA FRUTÍCOLA
dc.relationUID/ QUI/50006/2013—POCI/01/0145/FERDER/007265pt_PT
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s12649-021-01477-zpt_PT
dc.subjectApple barkpt_PT
dc.subjectSubcritical waterpt_PT
dc.subjectGC–MSpt_PT
dc.subjectHPLC–PDApt_PT
dc.subjectBioactivitypt_PT
dc.titleChemical Characterization and In Vitro Bioactivity of Apple Bark Extracts Obtained by Subcritical Waterpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleVALORIZAÇÃO DE RESÍDUOS DA INDÚSTRIA FRUTÍCOLA
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F97049%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F78845%2F2011/PT
oaire.citation.titleWaste and Biomass Valorizationpt_PT
oaire.fundingStreamSFRH
person.familyNameDelerue-Matos
person.familyNameRamalhosa
person.familyNamede Sá Barroso
person.familyNameMorais
person.givenNameCristina
person.givenNameMaria João
person.givenNameMaria de Fátima
person.givenNameSimone
person.identifier1598822
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.ciencia-id421A-C491-F45B
person.identifier.ciencia-idAF16-22E5-5EBD
person.identifier.ciencia-idB111-BBFB-F200
person.identifier.orcid0000-0002-3924-776X
person.identifier.orcid0000-0002-1951-3557
person.identifier.orcid0000-0001-9011-5992
person.identifier.orcid0000-0001-6433-5801
person.identifier.ridD-4990-2013
person.identifier.ridD-8293-2013
person.identifier.scopus-author-id6603741848
person.identifier.scopus-author-id35488836000
person.identifier.scopus-author-id25631648000
person.identifier.scopus-author-id7007053747
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaid421A-C491-F45B | Maria João Dantas Ramalhosa Ferreira
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
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