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New Quantum-Dot-Based Fluorescent Immunosensor for Cancer Biomarker Detection

2022Journal article Open access
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dc.contributor.authorSousa, Mariana P.
dc.contributor.authorPiloto, Ana Margarida L.
dc.contributor.authorPereira, Ana Cláudia
dc.contributor.authorSchmitt, Fernando
dc.contributor.authorFernandes, Ruben
dc.contributor.authorMoreira, Felismina T. C.
dc.date.accessioned2023-01-26T09:53:03Z
dc.date.available2023-01-26T09:53:03Z
dc.date.issued2022
dc.description.abstractCancer antigen 15-3 (CA 15-3) is a biomarker for breast cancer used to monitor response to treatments and disease recurrence. The present work demonstrates the preparation and application of a fluorescent biosensor for ultrasensitive detection of the cancer antigen CA 15-3 protein tumor marker using mercaptopropionic-acid-functionalized cadmium telluride (CdTe@MPA) quantum dots (QDs) conjugated with CA 15-3 antibodies. First, the QDs were synthesized by the hydrothermal route, resulting in spherical nanoparticles up to 3.50 nm in diameter. Subsequently, the QD conjugates were characterized by Fourier transform infrared spectroscopy (FTIR), UV absorption, and fluorescence. The interaction between the conjugates and the protein was studied by fluorescence spectroscopy in buffer and in 10-fold diluted commercial human serum. Calibration in spiked serum samples gave a detection limit of 0.027 U/mL, 1000-fold lower than the clinical limit for CA 15-3 (25 U/mL to 30 U/mL), indicating that this is an ultrasensitive technique. In addition, a rapid response was obtained within 10 min. The biosensor was selective in the presence of the interfering serum proteins BSA, CEA, and CA-125, with a maximum interference of 2% for BSA. The percent recovery was close to 100% with maximum relative standard deviation (RSD%) values of 1.56. Overall, the developed CA 15-3 biosensor provides a simple and sensitive method for ultrasensitive monitoring of breast cancer, as well as the ability to detect other molecules of interest in human serum matrices.pt_PT
dc.description.sponsorshipThis research was funded by Fundação para a Ciência e Tecnologia, I.P, grant number 2022.09032.PTDCpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/chemosensors10120518pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/21878
dc.language.isoengpt_PT
dc.relation2022.09032.PTDCpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAntibodypt_PT
dc.subjectCA 15-3 biosensorpt_PT
dc.subjectFluorescencept_PT
dc.subjectQuantum dotpt_PT
dc.titleNew Quantum-Dot-Based Fluorescent Immunosensor for Cancer Biomarker Detectionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue12pt_PT
oaire.citation.startPage518pt_PT
oaire.citation.titleChemosensorspt_PT
oaire.citation.volume10pt_PT
person.familyNameL. Piloto Cerqueira
person.familyNamePereira
person.familyNameSchmitt
person.familyNameFernandes
person.givenNameAna Margarida
person.givenNameAna Cláudia
person.givenNameFernando
person.givenNameRúben
person.identifier635792
person.identifier.ciencia-idAD1C-28E2-F781
person.identifier.ciencia-id0D1F-4090-E82A
person.identifier.orcid0000-0002-2374-2467
person.identifier.orcid0000-0003-4106-0766
person.identifier.orcid0000-0003-1006-6946
person.identifier.orcid0000-0001-8933-3984
person.identifier.ridG-5788-2018
person.identifier.scopus-author-id8527885100
person.identifier.scopus-author-id7202545664
person.identifier.scopus-author-id57640135700
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationbed0bc46-bcdd-4f89-8eed-ac6c7620029e
relation.isAuthorOfPublicationdefcf8db-528b-4f80-b8cc-6ff3533b1296
relation.isAuthorOfPublication50b71284-49d8-41ba-ab9f-e520725e6c81
relation.isAuthorOfPublication917f7713-0a35-4ef0-ae56-0784f7fc2187
relation.isAuthorOfPublication.latestForDiscoverydefcf8db-528b-4f80-b8cc-6ff3533b1296

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