| dc.contributor.author | Martins, Gabriela V. | |
| dc.contributor.author | Fortunato, Elvira | |
| dc.contributor.author | Fernandes, Helena R. | |
| dc.contributor.author | Sales, M. Goreti F. | |
| dc.date.accessioned | 2016-02-26T15:50:18Z | |
| dc.date.available | 2016-02-26T15:50:18Z | |
| dc.date.issued | 2014 | |
| dc.description | 1st ASPIC International Congress | pt_PT |
| dc.description | 1st ASPIC International Congress - Lisboa, 25-26 Novembro 2014 | |
| dc.description.abstract | Free radicals and other reactive species are constantly generated in vivo and can cause oxidative damage to biomolecules, a process that seems to play an important role at the origin of cancer. 8-Hydroxy-2'-deoxyguanosine (8-OHdG) is a major product of DNA hydroxylation and is considered a biomarker of damage caused by oxidative stress (OS). Thus, early diagnosis of OS biomarkers may be used as a fundamental tool in cancer prevention and in more efficient therapeutic strategies. For this purpose, a biomimetic sensor for 8-OHdG detection and quantification by Electrochemical Impedance Spectroscopy (EIS) is proposed herein. The biomimetic sensor was obtained by modifying a clean gold (Au) electrode with a OH-terminal thiol compound, followed by direct electropolymerization of phenol in the presence of 8-OHdG. The biomimetic/Au acted as working electrode, while glassy carbon and Ag/AgCl were used as counter and reference electrodes, respectively. Electropolymerization of phenol was performed by Cyclic Voltammetry (CV) over the potential range 0.2 to 0.9 V in pH 7.0 PBS buffer, enabling the formation of a non-conductive layer. Non-imprinted materials (NIM) were also performed by removing the template from the procedure and, then, the ability of the polymer to interact non-specifically with the template was measured. Preliminary results showed the development of a direct and label-free biomimetic sensor with good performance, stability and sensibility. In particular, only MIP material was able to rebind to the target molecule and produce a linear response against EIS on the range 0.010 to 10ng/ml. Overall, the biosensor described herein is simple, precise and may allow routine use for biological samples on-site. | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.22/7751 | |
| dc.language.iso | eng | en |
| dc.title | A biomimetic sensor for monitoring oxidative stress biomarker in point-of-care | pt_PT |
| dc.type | other | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | Lisboa | pt_PT |
| oaire.citation.title | 1st ASPIC International Congress - Lisboa, 25-26 Novembro 2014 | pt_PT |
| person.familyName | Martins | |
| person.givenName | Gabriela | |
| person.identifier.ciencia-id | 0219-87C9-5E5B | |
| person.identifier.orcid | 0000-0001-6739-1526 | |
| person.identifier.scopus-author-id | 55918092100 | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | other | pt_PT |
| relation.isAuthorOfPublication | 3a45eaa7-19aa-4321-8ad3-9fc1bb0374d7 | |
| relation.isAuthorOfPublication.latestForDiscovery | 3a45eaa7-19aa-4321-8ad3-9fc1bb0374d7 |
