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Electrocatalytic evaluation of DNA damage by superoxide radical for antioxidant capacity assessment

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dc.contributor.authorBarroso, M. Fátima
dc.contributor.authorde-los-Santos-Álvarez, N.
dc.contributor.authorLobo-Castañón, M. J.
dc.contributor.authorMiranda-Ordieres, A. J.
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorOliveira, M. Beatriz P. P.
dc.contributor.authorTuñón-Blanco, P.
dc.date.accessioned2013-11-27T17:01:42Z
dc.date.available2013-11-27T17:01:42Z
dc.date.issued2011
dc.description.abstractThe integrity of DNA purine bases was herein used to evaluate the antioxidant capacity. Unlike other DNA-based antioxidant sensors reported so far, the damaging agent chosen was the O 2 radical enzymatically generated by the xanthine/xanthine oxidase system. An adenine-rich oligonucleotide was adsorbed on carbon paste electrodes and subjected to radical damage in the presence/absence of several antioxidant compounds. As a result, partial damage on DNA was observed. A minor product of the radical oxidation was identified by cyclic voltammetry as a diimine adenine derivative also formed during the electrochemical oxidation of adenine/guanine bases. The protective efficiency of several antioxidant compounds was evaluated after electrochemical oxidation of the remaining unoxidized adenine bases, by measuring the electrocatalytic current of NADH mediated by the adsorbed catalyst species generated. A comparison between O 2 and OH radicals as a source of DNA lesions and the scavenging efficiency of various antioxidant compounds against both of them is discussed. Finally, the antioxidant capacity of beverages was evaluated and compared with the results obtained with an optical method.por
dc.identifier.doi10.1016/j.jelechem.2011.04.022pt_PT
dc.identifier.issn1572-6657
dc.identifier.urihttp://hdl.handle.net/10400.22/3008
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.ispartofseriesJournal of Electroanalytical Chemistry; Vol. 659, Issue 1
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S1572665711002104por
dc.subjectNADH electrocatalysispor
dc.subjectDNA damagepor
dc.subjectAntioxidant capacitypor
dc.subjectAscorbic acidpor
dc.subjectReactive oxygen speciespor
dc.subjectSuperoxide radicalpor
dc.titleElectrocatalytic evaluation of DNA damage by superoxide radical for antioxidant capacity assessmentpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage49por
oaire.citation.startPage43por
person.familyNamede Sá Barroso
person.familyNameDelerue-Matos
person.givenNameMaria de Fátima
person.givenNameCristina
person.identifier.ciencia-idAF16-22E5-5EBD
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0001-9011-5992
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id25631648000
person.identifier.scopus-author-id6603741848
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication3d701c85-4580-4614-be6d-5a1abc8cf729
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscovery3d701c85-4580-4614-be6d-5a1abc8cf729

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