Publication
Determination of the radiochemical purity of 99mTc-Tetrofosmin: comparision between five methods
| dc.contributor.author | Aguiar, Ana | |
| dc.contributor.author | Oliveira, Marta | |
| dc.contributor.author | Teixeira, Dalila | |
| dc.contributor.author | Cunha, Lídia | |
| dc.contributor.author | Metello, Luís F. | |
| dc.date.accessioned | 2013-10-16T23:11:46Z | |
| dc.date.available | 2013-10-16T23:11:46Z | |
| dc.date.issued | 2010 | |
| dc.description.abstract | GOAL: The manufacturing and distribution of strips of instant thin - layer chromatography with silica gel (ITLC - SG) (reference method) is currently discontinued so there is a need for an alternative method f or the determination of radiochemical purity (RCP) of 99m Tc - tetrofosmin. This study aims to compare five alternative methods proposed by the producer to determine the RCP of 99m Tc - tetrofosmin. METHODS: Nineteen vials of tetrofosmin were radiolabelled with 99m Tc and the percentages of the RCP were determined. Five different methods were compared with the standard RCP testing method (ITLC - SG, 2x20 cm): Whatman 3MM (1x10 cm) with acetone and dichloro - methane (method 1); Whatman 3MM (1x1 0 cm) with ethyl acetate (method 2); aluminum oxide - coated plastic thin - layer chromatography (TLC) plate (1x10 cm) and ethanol (method 3); Whatman 3MM (2x20 cm) with acetone and dichloro - methane (method 4); solid - phase extraction method C18 cartridge (meth od 5). RESULTS: The average values of RCP were 95,30% ± 1,28% (method 1), 93,95 ± 0,61% (method 2), 96,85% ± 0,93% (method 3), 92,94% ± 0,99% (method 4) and 96,25% ± 2,57% (method 5) (n=12 each), and 93,15% ± 1,13% for the standard method (n=19). There we re statistical significant differences in the values obtained for methods 1 (P=0,001), 3 (P=0,000) and 5 (P=0,004), and there were no statistical significant differences in the values obtained for methods 2 (P=0,113) and 4 (P=0,327). CONCLUSION: From the results obtained, methods 2 and 4 showed a higher correlation with the standard method. Unlike method 4, method 2 is less time - consuming than the reference method and can overcome the problems associated with the solvent toxicity. The remaining methods (1, 3 and 5) tended to overestimate RCP value compared to the standard method. | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/2362 | |
| dc.language.iso | eng | por |
| dc.publisher | Instituto Politécnico do Porto. Escola Superior de Tecnologia da Saúde do Porto | |
| dc.subject | 99mTc - tetrofosmin | |
| dc.subject | Thin-layer chromatography | |
| dc.subject | Quality control | |
| dc.title | Determination of the radiochemical purity of 99mTc-Tetrofosmin: comparision between five methods | por |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | International | por |
| oaire.citation.title | I International Congress of Health Gaia-Porto | por |
| person.familyName | Cunha | |
| person.givenName | Lídia | |
| person.identifier.orcid | 0000-0002-9260-8093 | |
| rcaap.rights | openAccess | por |
| rcaap.type | conferenceObject | |
| relation.isAuthorOfPublication | ed51e8c5-a1c2-40e6-b976-57af77446e1b | |
| relation.isAuthorOfPublication.latestForDiscovery | ed51e8c5-a1c2-40e6-b976-57af77446e1b |