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Automated lab-on-chip for the specific detection of invasive species through environmental DNA

2024Journal article Open access
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dc.contributor.authorElumalai, Monisha
dc.contributor.authorIpatov, Andrey
dc.contributor.authorGuerreiro, Joana
dc.contributor.authorPrado, Marta
dc.date.accessioned2025-01-15T16:16:42Z
dc.date.available2025-01-15T16:16:42Z
dc.date.issued2024
dc.descriptionThe authors thank the Confederación Hidrográfica del Guadalquivir (Spain) for collecting field samples. This work was supported by the Partnership Agreement with Confederación Hidrográfica del Guadalquivir (Spain) for the development of a system of early detection of the zebra mussel through analysis of environmental DNA and by the project Nanotechnology Based Functional Solutions (NORTE-01–0145-FEDER-000019), supported by Norte Portugal Regional Operational Programme (NORTE2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund.pt_PT
dc.description.abstractThe ability to detect nucleic acid sequences is revolutionizing the fast identification of specific organisms, particularly with Lab-on-a-chip systems. However, these often rely on complex processes, skilled personnel, and external control devices which limits automation. To address this, we present an automated, portable, and easyto-fabricate lab-on-chip that combines enzyme-assisted DNA signal amplification and optical detection for in-situ monitoring of zebra mussel invasive species in the environment. Zebra mussels DNA triggers enzyme-assisted signal amplification through hybridization, leading to a quantitative colorimetric response. The sensing performance exhibited enhanced sensitivity when increasing AuNPs diameter from 23.3 ± 1.6 to 67.4 ± 2.0 nm. Quantitative colorimetric approaches displayed an LoD of 0.5 pM, 19-fold increase in sensitivity when compared to naked eye. The system enabled to discriminate single nucleotide polymorphism (SNP) for concentrations from 130 pM and to successfully analyse Zebra mussel samples from the Guadalquivir River. Experiments were conducted by the user and an automated device. The accuracy was tested with a 60 pM sample which was pretreated externally and analysed by the automated system resulting in relative error of 16.8% and 13.4%, respectively. The automated system reduced the analysis time by 1 h and 20 min, great advantage for in-situ analysis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMonisha Elumalai, Andrey Ipatov, Joana Guerreiro, Marta Prado, Automated lab-on-chip for the specific detection of invasive species through environmental DNA, Sensors and Actuators B: Chemical, Volume 398, 2024, 134722, ISSN 0925-4005, https://doi.org/10.1016/j.snb.2023.134722.pt_PT
dc.identifier.doi10.1016/j.snb.2023.134722pt_PT
dc.identifier.issn0925-4005
dc.identifier.urihttp://hdl.handle.net/10400.22/27106
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSensors and Actuators: B. Chemicalpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0925400523014375pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectPlasmon couplingpt_PT
dc.subjectSignal amplificationpt_PT
dc.subjectDNA sensingpt_PT
dc.subjectNanoparticlespt_PT
dc.subjectColor sensorpt_PT
dc.subjectAutomated detectionpt_PT
dc.titleAutomated lab-on-chip for the specific detection of invasive species through environmental DNApt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleSensors and Actuators B: Chemicalpt_PT
oaire.citation.volume398pt_PT
person.familyNameElumalai
person.familyNameIpatov
person.familyNameGuerreiro
person.familyNamePrado Rodríguez
person.givenNameMonisha
person.givenNameAndrey
person.givenNameJoana Rafaela
person.givenNameMarta
person.identifierK-7365-2012
person.identifier.ciencia-id031D-1911-12B6
person.identifier.ciencia-id6B12-98A6-18BE
person.identifier.ciencia-idD312-F711-AE33
person.identifier.orcid0000-0002-4458-8737
person.identifier.orcid0000-0001-8523-4997
person.identifier.orcid0000-0001-8719-1978
person.identifier.orcid0000-0002-0529-5771
person.identifier.ridK-5881-2015
person.identifier.scopus-author-id35730167800
person.identifier.scopus-author-id7102374834
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication90a1bf4a-f3c3-4463-85da-c7719409ca5e
relation.isAuthorOfPublicatione478b741-4de9-4179-be9e-4bc44e2bf435
relation.isAuthorOfPublication94053d18-8ad3-4adc-8c44-859e7e572b91
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relation.isAuthorOfPublication.latestForDiscovery90a1bf4a-f3c3-4463-85da-c7719409ca5e

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